Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,159	T→A	intergenic (‑177/+426)	ompF ← / ← asnS	outer membrane porin F/asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,159	0	T	A	100.0%	34.4 / NA	14	intergenic (‑177/+426)	ompF/asnS	outer membrane porin F/asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/5);  total (9/5)

CAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAG  >  NC_000913/986946‑987361                                                                                                                                                                                                                      |                                                                                                                                                                                                           cAGGGACGATCACTGCCAGCATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAACGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAttt                                                                                                                                                                       >  1:137691/1‑251 (MQ=255)      aCGATCACTGCCAGAATATTGCGCTTCATCATTATTTCTTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGATCTGTCAATAAATCTTTACGGAAATATTGATGAGAGTTTGGTGTCTTTCTGTGTCTGCAGGCATCTTTCCATTCACACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGAAAAATTTAAg                                                                                                                                                                    >  1:367581/1‑249 (MQ=255)                                                                  gactccTGCCACTGCCGTCAATAAGTTCTGTCAAGAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTTCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAAGATGTAACCAAAAGTAAAATTTAAGTAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATc                                                                                                      <  1:496742/247‑1 (MQ=255)                                                                      ccTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTAtt                                                                                                  >  1:205888/1‑251 (MQ=255)                                                                            aCNNNNNTCANTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCaa                                                                                                                                                                                                       <  2:306131/144‑1 (MQ=255)                                                                               gCCGTCAATAAGTTCTGTCAATAAAAATTTACGTAACTATTGATGAGAGTGTGGTGTCTTTAGGTGTCTGCAGTCATCTTGCCACTCAAACTAACGTTTCTCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTc                                                                                                                                                                                                         <  1:458302/139‑1 (MQ=255)                                                                                                                        gatgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAACCTATCGTTTCGACACCGTGACAGTGCTACAAAGATCAATCTTTGGATTCAACAAGAAAAAatat                                                                                                                                                                                          >  1:241356/1‑113 (MQ=255)                                                                                                                                                     cAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTta                                                                                                >  1:387428/1‑174 (MQ=255)                                                                                                                                                                 cATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGaaa                                                            >  1:347023/1‑198 (MQ=255)                                                                                                                                                                 cATNCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACNGTCNTATTNCCNTAGAGACGTGATGATNTTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAANNTNNNTNAACGGaaa                                                            <  2:347023/198‑1 (MQ=255)                                                                                                                                                                      aaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAg  <  1:335030/251‑1 (MQ=255)                                                                                                                                                                           aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAAtcttc                >  1:422933/1‑232 (MQ=255)                                                                                                                                                                                               gTGCTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGAc        >  1:206383/1‑220 (MQ=255)                                                                                                                                                                                                  cTACAAAGATAAATATTTGGATTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAAAACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTATATTAACTAAACGGAACTTTTGTTTCTGATGGAAACTTTATCGACCTGGca                          >  1:504318/1‑199 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                           CAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAG  >  NC_000913/986946‑987361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑