| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,603,828 | G→A | A671T (GCC→ACC) | hyfB → | hydrogenase 4 component B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,603,828 | 0 | G | A | 95.0% | 49.0 / ‑7.1 | 20 | A671T (GCC→ACC) | hyfB | hydrogenase 4 component B |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (8/11); minor base T (0/1); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
GCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGAT > NC_000913/2603629‑2604070 | gCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGNc < 1:395340/251‑1 (MQ=255) ngAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATg > 1:300558/2‑219 (MQ=255) gATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTATCACAGGCCGTGACTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTccn < 1:104887/251‑2 (MQ=255) nnnnnntCNNNNNCATNGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATg < 2:300558/144‑1 (MQ=255) aCCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACGGGTTGTTCGGCTAATCGCTATTTCCGTCTAAg < 1:406951/112‑1 (MQ=255) ncTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACAATGAGACAAACTCttt > 1:299268/2‑129 (MQ=255) gCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGc < 1:140282/209‑1 (MQ=255) aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCCCTTTTTACGGGTATTTCCCGGCATATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTa < 1:205071/250‑1 (MQ=255) gCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTGTGTGGTCGCCGCACTGGTTGTGATGCTAATCACTATTACCGTCTAAGGAAATCACCATg > 1:472555/1‑98 (MQ=255) gcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCAGGAGACAAACTCTTTGCGACGGATAGCTGGTCATGGTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAccc < 1:185708/157‑1 (MQ=255) gACTTTCGTCTCTATTGCCTGGATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGt < 1:240135/111‑1 (MQ=255) gTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTNNGACgg > 2:13100/1‑95 (MQ=255) gTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcc < 1:13100/147‑1 (MQ=255) cTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcg < 1:163395/172‑1 (MQ=255) tATGTGGTAGACGCACTGGTTGTGCTNCTAATCNNTCNTACCGTCTA‑GGAAATAACCNTGNNNCNNNCNCTTTGNGANNGATATCTGGNCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTANGGGTATTTCNNGGCAGATACGCGCGCGTATGCNCTCNNGNNGNGGGNCg > 2:106874/1‑192 (MQ=255) gTCGCCGCACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCGCCATGAGACAAACTCTTTGCGACGGATATCTGGGCATGTGTGCGTTAGCACAGGCCGTGATTctgct < 1:206113/117‑1 (MQ=255) cACTGGTTGTGCTGCTAATCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTNNGcac > 2:7559/1‑94 (MQ=255) gctAATCGCTATTACCGTCTA‑GGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCGGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCg < 1:106874/249‑1 (MQ=255) tCGCTATTACCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAAGGATATCTGGTCATTTTTACGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCAGGGGATCTGGAAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGCAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGAt > 1:3047/1‑251 (MQ=255) cTATTACCGTCTAAGGAAATCACCATGAGACAAAATCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGa > 1:174041/1‑235 (MQ=255) | GCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGAT > NC_000913/2603629‑2604070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |