Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,086,235	G→A	F18F (TTC→TTT)  E17K (GAA→AAA)	yqgG ← yqgC →	protein YqgG protein YqgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,086,235	0	G	A	100.0%	41.7 / NA	19	F18F (TTC→TTT)  E17K (GAA→AAA)	yqgG yqgC	protein YqgG protein YqgC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/6);  total (13/6)

GGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCAAAAGATAAATACGCAGAATGCCGGCATTGTC  >  NC_000913/3086038‑3086476                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ggTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCGTGTTTTTACAAGAGCGCCCTTGGTCAATCCTCAGTAACGGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACAGCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTg                                                                                                                                                                                                                            <  1:282016/221‑1 (MQ=255)                           nacaTAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGgccgcc                                                                                                                                                                    >  1:148336/2‑251 (MQ=255)                                  ccGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGgccgcn                                                                                                                                                                    <  1:62246/244‑2 (MQ=255)                                   cNAACGTAAGAGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGACCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGGATAACATCGGCAGGAATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGAAAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGttc                                                                                                                                                               >  1:392289/1‑247 (MQ=255)                                             tgtgAAAGTTCGGCGAAACCACGATATTTCTCTTGTTTTTACAAGAGCGACCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGAATGCAAAGCAGAGATGCAGAGCGCGGGAAACGAATCTTCACCAGAACGGCGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAAc                                                                                                                                                       >  1:270984/1‑246 (MQ=255)                                                          cGAAAACACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCATCAGTCACTGTCACCAGCTCTTGAATCCTGAGACGCGACGAGATGGGTATAACATCGGCAGGTATGCAAAGCAAAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGGGAGACAGGTTAAGCAGCAGACAACGGATCATTATTTCGTCTCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCACAACTCTg                                                                                                                                     >  1:337848/1‑251 (MQ=255)                                                               ccACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTc                                                                                                                                >  1:349220/1‑251 (MQ=255)                                                                               gTTTTTACAAGAGCTCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTGGAATCCTGAGAAGCGCCGAGATGTGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCAcc                                                                                                                                                                                                                                     <  1:71032/134‑1 (MQ=255)                                                                                              cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAAc                                                                                                                                                                                                                                                >  1:32633/1‑108 (MQ=255)                                                                                                          ccTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAACAGCGATGCAGTGTGCGGGACACGAATCTTCACCa                                                                                                                                                                                                                                    >  1:462632/1‑108 (MQ=255)                                                                                                             cAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGc                                                                                                                        >  1:180172/1‑213 (MQ=255)                                                                                                                             ctctTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCCGAGATGCAGAGTGCGGGAAACGaa                                                                                                                                                                                                                                             >  1:453517/1‑80 (MQ=255)                                                                                                                                      ccTGAGAAGCGCCGAGATGGTTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCCCCAGAACTGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCTTTTtat                                                         <  1:15932/251‑1 (MQ=255)                                                                                                                                      ccTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACAAGCACGGTga                                                                                                                                                                                                                           >  1:370514/1‑89 (MQ=255)                                                                                                                                                    agaTGGGTNTNNNANNCGCAGGTATGCAAAGCAGAGATGCAGAGGGCGGGAAACGAATCTNCACCAGAACGGGGGGNCCNGTTANGCANCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGGTAAGACg                                                                                                                                                          >  2:343663/1‑140 (MQ=255)                                                                                                                                                    agaTGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTGCACCAGAACGTTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACg                                                                                                                                                          <  1:343663/140‑1 (MQ=255)                                                                                                                                                         ngTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGaaa                                       >  1:94448/2‑250 (MQ=255)                                                                                                                                                                                          gCAGAGTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGc                                            <  1:171374/212‑1 (MQ=255)                                                                                                                                                                                               gTGCGGGAAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTCTCACCTCCACGGCCGCCTGTTAAGACGCACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCAAAAGATAAGTACGCAGAATGCCGGCATTGTc  >  1:270829/1‑249 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCAAAAGATAAATACGCAGAATGCCGGCATTGTC  >  NC_000913/3086038‑3086476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑