| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,135,729 | C→T | T632I (ACT→ATT) | katG → | catalase/hydroperoxidase HPI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,135,729 | 0 | C | T | 92.9% | 31.8 / ‑3.7 | 15 | T632I (ACT→ATT) | katG | catalase/hydroperoxidase HPI |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (6/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCTGGTGTTTGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAA > NC_000913/4135562‑4135957 | tGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGCGTATTGAGc < 1:239565/190‑1 (MQ=255) gACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTTCGCTGGTGGGCGGCATGCGTGTACTGGGGGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGAc < 1:81147/141‑1 (MQ=255) tCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGNg < 1:396271/145‑1 (MQ=255) tCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGCGTATGGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAGAGAGCTGTTc < 1:422399/225‑1 (MQ=255) cACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgt > 1:244797/1‑221 (MQ=255) gATCGACAAAGCACAGCAACTGACGCGGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCGGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGc < 1:208178/250‑1 (MQ=255) nnnnnnnngntcnNGAAANGACTGCGCTGGTGGGCGGCATGCGNGTACTGGGTGCCAACTTCGATGGCAG‑CAAAANCNGCGTCTTCAGTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGANCTTGCTGGATNTGCNNTACGNGNGNAAATCTACCGACGAATCGAAAGAGCTGTTNGAAGGCCGTGACCGTGAAACCGGNNAANNNAANNTTAct < 2:365277/210‑2 (MQ=255) naCCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGAGCACTGGGTGCCACCTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGaa > 1:63603/2‑196 (MQ=255) ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGATGCCAACTTCGATGGCAG‑CAAAAACGGAGTCTTCATTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCATGAACTTGCTGGCTATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCTGGTGTTTGGTTCTAACTCCGTCCt > 1:241835/1‑251 (MQ=255) ggAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGGCAAAAA‑GGCGTCTTCCTTGACCGCGATGGCGAATTGAGCACTGACTTCTTCGTGAACTTGCTGGATATGCGTTCCGAGTGGAAAGCGACCGACGAATCGAAGGCGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACg > 1:365277/1‑208 (MQ=255) gcggcATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCATTGACCGCATTGGCGTATTGCGCAATGACTTCTTCGTGCACTTGCTGGATCTGCGTTACGAGTGGAAAGCGACCGACGAATc > 1:88772/1‑136 (MQ=255) tGGCAG‑CAAAAACGGCTTCTTCATTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGTATATTCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGt < 1:15054/116‑1 (MQ=255) gcag‑caAAAACGGCGTCTTTACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGACGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGACAGCCGTGCGGATCTGGTGTTTGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTaa > 1:425796/1‑250 (MQ=255) ggCGTCTGCATTGACCNCGTTGGCGTANTGANNAATGNCNTNTTCGTGAACTTGCTAGATATGCGTTACGANTGGAAAGCGACCGACGAATCGAANNAGNNNTTNNAAGGc < 2:381873/111‑1 (MQ=255) ggCGTATTCATTGACCGCGTTGGCGTATTGAGCAATGACTTATTCGTGAACTTGCTGGCTATGCGTTACGTGTGGGAAGCGACAGACGAACCGCAAGAGATGTTCGAGGGc > 1:381873/1‑111 (MQ=255) | TGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAG‑CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCTGGTGTTTGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAA > NC_000913/4135562‑4135957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |