Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,070,014	A→G	L114P (CTG→CCG)	rutF ←	FMN reductase RutF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,070,014	0	A	G	100.0%	32.9 / NA	14	L114P (CTG→CCG)	rutF	FMN reductase RutF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/7);  total (7/7)

GTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGAC  >  NC_000913/1069816‑1070184                                                                                                                                                                                                       |                                                                                                                                                                           gTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGAGAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTTACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGggcggc                                                                                                                        <  1:67797/251‑1 (MQ=255)           ccGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCgg                                                                                                              <  1:388605/251‑1 (MQ=255)                                                           gcATCAGCGCGTGATAACTGCGATCTAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGc                                                              <  1:386375/251‑1 (MQ=255)                                                              tcggcgcGGGATAACTGCGATCAAACCACACCAGCCCGTAGGGTTTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCCCCACCTGGCGGAAACGACAATCAAGCGAAACCGGACCGTCGGCCAGTCGCGGGCATGCGATGACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGgtgt                                                                                                           <  1:476933/200‑1 (MQ=255)                                                                   gcgTGATAACTGCGATCGCACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGTCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                                           >  1:42326/1‑150 (MQ=255)                                                                        agaacggCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCGTCGATGGCGAAAAACAGAATGGCGTGGGTGCCGACGCTCACCACCGGTCTGAGACGGCAGTCAAAAGAAACCGGAGACGCGGCCAGTTGCGGGCATCCgg                                                                                                                                                 <  1:262014/149‑1 (MQ=255)                                                                            cTGCGATCGAACCACACCAGCCCGTAGGGTGTGGCGTGACGATGAATCGCTTCGATGGAGCAAAACAGAATGTCGTGGGTGCCGACGATCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCCAAGCGGTGTTc                                                                                                         >  1:192113/1‑191 (MQ=255)                                                                                     aaCCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCa                                                                                                                                                                >  1:412688/1‑127 (MQ=255)                                                                                                nccGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCCACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGcc                                                                                                                                                                       >  1:56989/2‑109 (MQ=255)                                                                                                        gtgtgctgtgACGATGACTCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGggcggcggc                                                                                                                     >  1:403660/1‑151 (MQ=255)                                                                                                                       gAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACCAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAACAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACCGCGTTCGATTTTCTTTGAATGCAGGCCAgacggac  >  1:73642/1‑251 (MQ=255)                                                                                                                         aTCGCTNNGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                                           <  2:42326/96‑1 (MQ=255)                                                                                                                                     nnnnnnaaNNNNNTGTNGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACAGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTgccg                                                                                         <  2:314796/144‑1 (MQ=255)                                                                                                                                                                                     cGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAACGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTcc                                                                 >  1:161517/1‑126 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                           GTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGAC  >  NC_000913/1069816‑1070184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑