Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	60.2 / NA	20	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

CATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATAT  >  NC_000913/2484587‑2485072                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGACGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGAccc                                                                                                                                                                                                                                             >  1:440871/1‑249 (MQ=255)     ccGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCGAAAAGCAATGGACGCGCTTGCAGAAGTTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTATTAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATg                                                                                                                                                                                                                                         <  1:497877/251‑1 (MQ=255)                                            attTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTTTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGGTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCg                                                                                                                                                                                                   <  1:64657/250‑1 (MQ=255)                                                              gagCGTTAAATATCAAATTAAAACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAg                                                                                                                                                                                              >  1:120161/1‑237 (MQ=255)                                                                                                              aaGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCAcc                                                                                                                                                                                                                        >  1:11456/1‑163 (MQ=255)                                                                                                               aGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTTCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCAc                                                                                                                                                                                                                                                  <  1:243940/136‑1 (MQ=255)                                                                                                                      gACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTATCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                                                                         >  1:506266/1‑234 (MQ=255)                                                                                                                                      gTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATGACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAGTATCTCTTTTACAAATTTATATCAGGCATTa                                                                                                           <  1:453828/248‑1 (MQ=255)                                                                                                                                                                       cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAATCACCTTTCACGACTCAATGCGACCGCTTACCTCACCaa                                                                                                                                                                                                                      >  1:20360/1‑108 (MQ=255)                                                                                                                                                                       cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACAATTGATAATTACATTTCCGGCGCTGGTAAACACCTTTCACGACTCAATGCGACCGCTTAcc                                                                                                                                                                                                                             >  1:338538/1‑101 (MQ=255)                                                                                                                                                                          cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCTCCTTTCACTACTCAATGCGACCGCTTTCCTCACCAAAACCAGTAAATAGTTCTCGGGTAGCAAATTACTCCCCAGACGAGGTAATTCATCGATCATTTCCAAAAGGAACAATTATCTCTTTTACAAATTTATATCAGGCa                                                                                                              <  1:454652/209‑1 (MQ=255)                                                                                                                                                                              gccTCATAATAATGACATTGCTGCAACCAAACCATTTATAATTTCCTTGCCGGCGCGAGCAACCGCCTTTCACGACTCAATGCGACCGCTTACCTCAc                                                                                                                                                                                                                         <  1:241233/98‑1 (MQ=255)                                                                                                                                                                                         atGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCNNAACCAGNna                                                                                                                                                                                                             >  2:3589/1‑99 (MQ=255)                                                                                                                                                                                         atGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAg                                                                                                                                                                                              <  1:3589/114‑1 (MQ=255)                                                                                                                                                                                                     cAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtctc                                                                                                <  1:1857/196‑1 (MQ=255)                                                                                                                                                                                                        ccaaaccaTTGATAATTACATTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAACCCAGTACATATTGCTCGGGTCGCAAATTCCCCCCCAGACGa                                                                                                                                                                           >  1:410827/1‑118 (MQ=255)                                                                                                                                                                                                             ccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACATCACAAAAACCAGTAAATATTGCTCg                                                                                                                                                                                                   >  1:366513/1‑89 (MQ=255)                                                                                                                                                                                                                 tGATAATTACCTTTCCGGCGCTGGTAACCCCCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGan                                                                                                                                                                          <  1:405780/110‑2 (MQ=255)                                                                                                                                                                                                                          ccTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACTTCACCCAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAACAGCAACAATTATCTCTTTTACAACCTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                                                                  >  1:487239/1‑189 (MQ=255)                                                                                                                                                                                                                                            ccaccTTTCACGAATAAATGCGAGCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAatat  >  1:384498/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATAT  >  NC_000913/2484587‑2485072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑