Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,539,633	G→A	G750D (GGT→GAT)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,539,633	0	G	A	100.0%	44.7 / NA	18	G750D (GGT→GAT)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/7);  total (11/7)

GCATCAGGACGGCCTGGTTCACATCTCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGC  >  NC_000913/3539400‑3539863                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       gCATCAGGACGGCCTGGTTCACATCTCTTCATTGTCGCCCAAGTTTGTGGAAGCTCCGCATACCGTGGTGCAAGCGGGCGCCATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTACACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAAACGCCCGGCAGCCAAACCACGCGATCGTGAAg                                                                                                                                                                                                                                >  1:483440/1‑242 (MQ=255)                                            tttGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAgn                                                                                                                                                                                                            <  1:129016/219‑2 (MQ=255)                                              tgtgGAAGATCCGCATTCCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGc                                                                                                                                                                           <  1:21337/250‑1 (MQ=255)                                                                    gtgAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCNTGGNGAAANCANCGCTCGTCGCGGCGGNGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGNNANNNTNAAGCGCAg                                                                                                                                                                                                                           <  2:349519/180‑1 (MQ=255)                                                                    gtgAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAg                                                                                                                                                                                                                           >  1:349519/1‑180 (MQ=255)                                                                          gCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCACAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACg                                                                                                                                                                   >  1:209521/1‑230 (MQ=255)                                                                                                                        ntAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGAAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                                                                                                                                                                                    >  1:44118/2‑167 (MQ=255)                                                                                                                                                         agcagcCTGGCAAAACCAACGCTCGGCGCGGCGGCGGTAAGTAACGCCCTCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAgc                                                                                                                                                                                                                               <  1:242278/91‑1 (MQ=255)                                                                                                                                                                 ggCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTATTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCa                                                                                                   >  1:409898/1‑207 (MQ=255)                                                                                                                                                                                                       nccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTc                 >  1:403944/2‑251 (MQ=255)                                                                                                                                                                                                       cccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaac                                                                               >  1:390610/1‑189 (MQ=255)                                                                                                                                                                                                        ccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGGTGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCACGGACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaac                                                                                   >  1:79486/1‑184 (MQ=255)                                                                                                                                                                                                                  cccGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACTGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACTCCTTGCCTGACATAAAAGTGCCGGAGAATATCGCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTc      <  1:357486/250‑1 (MQ=255)                                                                                                                                                                                                                        cGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGACATCGTAGGGCAGATACGGCGTTCACGCCGCCTCTGGCaac                                                                                   >  1:215951/1‑168 (MQ=255)                                                                                                                                                                                                                         ggCATCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGGTAAACGCCCGTACTGGCCTACGGTTCGAAGTTGCACGAAATGGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACAGAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGc  <  1:359558/248‑1 (MQ=255)                                                                                                                                                                                                                              ncAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTg                                                                                                                                 >  1:315084/2‑116 (MQ=255)                                                                                                                                                                                                                              ccAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTg                                                                                                                                 <  2:315084/116‑1 (MQ=255)                                                                                                                                                                                                                                aaaCCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACCATGGGCAAAAAACGTTAAACGcc                                                                                                                                                          >  1:253682/1‑89 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       GCATCAGGACGGCCTGGTTCACATCTCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGC  >  NC_000913/3539400‑3539863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑