| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,320,907 | C→T | D160N (GAT→AAT) | trpD ← | anthranilate synthase subunit TrpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,320,907 | 0 | C | T | 89.5% | 48.5 / NA | 19 | D160N (GAT→AAT) | trpD | anthranilate synthase subunit TrpD |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/9); minor base G (0/2); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTCTGCGCCTGATACAGTTTTTCCAGAATCGGTTGCAGCGTGTTGGCTGGCTCTAGTTTCTGCTGCGCCCAGGCCAGCGTTTGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATCGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACC‑GTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGC > NC_000913/1320679‑1321140 | ccAGTTGTTCCGGCTTCAGCCCGCCACGCCCCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTCTGCGCCTGATACAGTTGTTCCAGAATCGGTTGCAGCGTGCTGGCTGGCTCTAGTTTCTGCTGCGCCCAGGCCAGCGTTTGTTCCATCAGGCGAGCGCCCTTGGTGGTGAGAATGGAGTCCGGATGGGACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCGCCATg < 1:262276/251‑1 (MQ=255) aGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGAGTCTGCGCCTGATACAGTTTTTACAGAATCGGATGCAGCGTGTTGGCTGGCTCTAGTTTCTGCTGCGCCCAGGCCAGCGTTTGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGCACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGtt > 1:109427/1‑251 (MQ=255) caccacCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTCTGCGCCTGATACAGTTTTTCCAGAATCGGTTGCAGCGTGTTGGCTGGCTCTAGTTTCTGCTGCGCCCAGGCCAGCGTTTGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAAc > 1:116801/1‑251 (MQ=255) gnnnnnctCNGGTTTCTGCTGCGCCCAGGCCAGCGTTGGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTaa < 2:303152/143‑1 (MQ=255) gctgcGCCCAGGCCAGCGTTGGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATTGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATGGTGACGTACTGCCATCACCATGCCATTGAAATGGGCGTGGATGTTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCATCGGCAGCTGGTTTGTTAATCCAGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCt < 1:449197/251‑1 (MQ=255) agacgtgggctctgccgttcgcagcagGGGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAATCCGTCCGTAATGTTACTGCCAACCAGCGACTGATAACGCGCCACCGGCAGCGGTTTTTTTAATCCGGCAAACATCGCCTGACCGTCAGGTTCAATGCTGGAGGCTTTAc < 1:203156/230‑1 (MQ=255) ncccAGGCCAGCGTTTGTTCCAGCAGGTGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGc > 1:99718/2‑209 (MQ=255) gcCCAGGCCAGCGTTTGTTCCAGCAGGCGAGCGCCCTGGGTGGGGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGNNTTGTGANNt > 2:5071/1‑99 (MQ=255) cAGGCCAGCGTTTGTTGCAGCAGGCGAGCGCCCTGGGTGGTGAGAATTGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGc < 1:322871/101‑1 (MQ=255) nagcagGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACCAACGCGATCCGCATTGCGACGTCCTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATACCGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTgg > 1:55989/2‑221 (MQ=255) cagcagGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGTCGTTGATGGTTAAACCGTCCGGAATGTTACTg < 1:425080/139‑1 (MQ=255) ggtggtGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACC‑GTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGt < 1:5071/251‑1 (MQ=255) gnATTNCNNANNGAANTGGAATCCACAAACGCGATCCNNATGGTGACGNACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATNTTACTGCCANNCANCGAGTNANNNCNNNCCNCCGGCAGCGGGTTTGTGAANCCNNCAAACANCGCCTGACCGTCATGTTCAATGCTgg < 2:116801/191‑1 (MQ=255) naNTNCGGATGGAACTGGAATCCACAAACGCGATNCGCATTGTGACGTACTGCCATCANNATNNNAGNNAAATg < 2:366389/73‑1 (MQ=255) gATTCCGAATGGAACTGGAATCCACAAACGCGATCCGCATTCTGACGTAATGCCATCACCATGCCATTAAAATg > 1:366389/1‑74 (MQ=255) gATGGAACTGGAATCCACAAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAg > 1:431611/1‑185 (MQ=255) ggAATNCACAANCNNGATCCGCATTGTGACGTACNGCCATCACCATGCCANTAAANTGGGCNTTNa > 2:196885/1‑66 (MQ=255) ggAATCCACAAACGCGATCCGCATTGTGACGGACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACC‑GTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCg < 1:196885/230‑1 (MQ=255) caAACGCGATCCGCATTGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCCGCATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGATGCTTTACCAGTG‑AGAATTTCGCCCTCCTGACCGACATAGCCCCCGTAAGGTTCGACAATCGCCTGATGTCCGAGGCAAATGc < 1:381993/250‑1 (MQ=255) | CCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTCTGCGCCTGATACAGTTTTTCCAGAATCGGTTGCAGCGTGTTGGCTGGCTCTAGTTTCTGCTGCGCCCAGGCCAGCGTTTGTTCCAGCAGGCGAGCGCCCTGGGTGGTGAGAATGGATTCCGGATGGAACTGGAATCCACAAACGCGATCCGCATCGTGACGTACTGCCATCACCATGCCATTAAAATGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACC‑GTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGC > NC_000913/1320679‑1321140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |