Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	100.0%	26.2 / NA	12	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

CCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGC  >  NC_000913/2410223‑2410663                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     cccAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                              >  1:255514/1‑237 (MQ=255)                     cGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATGCCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAg                                                                                                                                                                                           <  1:219034/236‑1 (MQ=255)                                                                                                                                           aaTGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTAGCCGCGCAATACCATCGGCTCCAGCAATTGCTAACTACAAAATTGCCGGACATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAAcgcaa                                                                                                                       >  1:240891/1‑184 (MQ=255)                                                                                                                                                  tctcTGCCATGCCCACATCAAGGACCTGGTCATAAAAATACGCGCCGCGCAAGACCATCGTCTCCAGCAAGTGCGGACGACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGc                                                                                                                                                                             <  1:248807/125‑1 (MQ=255)                                                                                                                                                      tGCCATGCCCACATCAAGGGCCTGGTCAGAAAAAGACTCGCCGCGCAATACCACCGGCCCCAGCAATTGCTCACTACAAAAGTGCCGGAGATCGACAGCACCCGCAGACATACCAATAAGCAAAACTTCACGCTCGCGAAATTCAGAAACCCCATTAACGTTCACGATACCGCGACGAAAACGCCGCCAGCGTTCaa                                                                                                 <  1:219440/197‑1 (MQ=255)                                                                                                                                                             nccACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATCCCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAAcgc                                                                                                                         >  1:93246/2‑166 (MQ=255)                                                                                                                                                                          ccTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCCGCAATTGCCCACTAGAAAATTGCCGGTTGTCGACATCCGCCGGGGACATATCAATAAGCAAAACGTCGCGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGACAACGCCGCCAGCGt                                                                                                     >  1:466274/1‑173 (MQ=255)                                                                                                                                                                              gTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCACTTGCTCACTACACAATTGCAGGAGATCgcc                                                                                                                                                                                                      >  1:261229/1‑70 (MQ=255)                                                                                                                                                                                naGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTc                                                                                                                                                      >  1:130287/2‑118 (MQ=255)                                                                                                                                                                                cAGAANAATACNCNNCGCGCAATACCCTCGGCTCNAGCAATTGCTCACTANAAAANTGCCGNAGNt                                                                                                                                                                                                          >  2:195534/1‑66 (MQ=255)                                                                                                                                                                                              ccgcGCAATACCATCGGCTCCAGCAATTGCTCCCTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCNACGNNNNngcnnnnnn                                                                                                        >  2:314828/1‑144 (MQ=255)                                                                                                                                                                                               cgcgCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATGGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTcgn  <  1:314828/251‑2 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGC  >  NC_000913/2410223‑2410663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑