Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	33.4 / NA	13	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/6);  total (7/6)

CGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGAT  >  NC_000913/1301775‑1302228                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             cGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTTCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGTCTATTGCTTGTGAAGTTATCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGc                                                                                                                                                                                                             <  1:377184/251‑1 (MQ=255)                         ccAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAACAGATTGGGTCGTAAACGAACGACTCGTTCTTGAACGCAGCCCGACCTCCTGGAACAACGCGAAAACCGTTATTAACCAGGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGa                                                                                                                                                                                       >  1:224389/1‑249 (MQ=255)                         ccAGCATGATAATATCGTCACCAACGGGGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAACCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGa                                                                                                                                                                                       >  1:120143/1‑249 (MQ=255)                                                                                         ntCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGGTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTAACGATGTCAAACGCTACCGTAGTGGTGAAATCGACTTGACGTATAACAGCATGCCGATAGAATTGGTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaaaaac                                                                                                                                      >  1:297452/2‑234 (MQ=255)                                                                                                                   cTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACAATGTGCGTGTGCGTACCGCACTg                                                                                            >  1:69335/1‑250 (MQ=255)                                                                                                                      gAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGATACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAACTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAAc                                                                                                                    >  1:360871/1‑223 (MQ=255)                                                                                                                           aCGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTCCCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCTATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTGCACGTTGATCCATACCTGTGCACTTACTAGTACGAAAGTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGa                                                                                   <  1:484796‑M1/251‑2 (MQ=255)                                                                                                                                                             gCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAACTCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTa                                                                                                                                                      >  1:445474/1‑150 (MQ=255)                                                                                                                                                                 aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAATAGATCCCGGAcn                                                                                                                                                                                        <  1:125563/112‑2 (MQ=255)                                                                                                                                                                                 ccGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGCAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGaccgcgatatcattgttcatacagtgaaagcgcag                                                 >  1:291532‑M1/1‑196 (MQ=255)                                                                                                                                                                                   gANNNNNACCNCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaccaac                                                                                                                                      <  2:297452/144‑1 (MQ=255)                                                                                                                                                                                                           aaaatCGACATGTCGTAAAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAATAGATCCGGGACGAAGTCCACGTGGATCCATACTTGTGCACTTCTTTTTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGaccgcgatatcattgttaataaagtgaaagcgcagggcaacatgcccgcctatggttacactccaccgtatactgatggcg   <  1:446066‑M1/250‑82 (MQ=255)                                                                                                                                                                                                            aaaTCGACATGACGTAAAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGaccgcgatatcatggttaataaagtgaaagcgcagggcaacatgcccgcctatggttacactccaccgtatactgatggcgc  <  1:350637‑M1/251‑83 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             CGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGAT  >  NC_000913/1301775‑1302228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑