Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	100.0%	56.1 / NA	21	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/12);  total (9/12)

GGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGT  >  NC_000913/4510020‑4510439                                                                                                                                                                                                                           |                                                                                                                                                                                                          ggAGAAGGACGGGTCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATc                                                                                                                                                                            >  1:398533/1‑250 (MQ=255)            ggCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACTCCGGCTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTACAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATcc                                                                                                                                                                <  1:75965/251‑1 (MQ=255)                    tGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGGATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCTCACTTTTTAGAAAAATCGATGGTCAGAGACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGAGTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAg                                                                                                                                                         <  1:450720/250‑1 (MQ=255)                                                   gggTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGAGTAGCAATGCCGTTCCAGGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCg                                                                                                                           <  1:324873/249‑1 (MQ=255)                                                         gaCACCGACTCATGAAGATATCATTGATAACAATCGATCCATAAACTGGCGTGTATACAGTGGCTGCAGGTGATTAAGCAAGCTGCACCTACCTACGTAGTCCCAAACGCACACTTTTTAGAAAAATCGCAGGTCAGAACCTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGCTTTACGTTGGCGTCTGATCATTGATTTATCCTCAACAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                                   >  1:364226/1‑251 (MQ=255)                                                                                                        ggCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCATAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAAc                                                                                                                                   <  1:81361/188‑1 (MQ=255)                                                                                                                                gATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGCTTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTg                                                                                        >  1:479265/1‑207 (MQ=255)                                                                                                                                    aaGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGTACCAg                                                                                                                                <  1:385246/163‑1 (MQ=255)                                                                                                                                             gCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAtttt                               >  1:182836/1‑251 (MQ=255)                                                                                                                                                      taAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTGACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGcc                                                                                                                                                       <  1:454690/122‑1 (MQ=255)                                                                                                                                                       aaGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTg                                                                            >  1:194908/1‑196 (MQ=255)                                                                                                                                                              agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTc                                                       >  1:260259/1‑210 (MQ=255)                                                                                                                                                              agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                     <  1:263687/212‑1 (MQ=255)                                                                                                                                                                 aaaCGCACACTTTTTAGAAAAATCGATGGTCATAAACTGGATTAGCAATTTCGTTCCAGGGTTGCTTTTGATTTTCGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAAcc                                                                                                                                  <  1:323159/132‑1 (MQ=255)                                                                                                                                                                        cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAgg    <  1:347348/251‑1 (MQ=255)                                                                                                                                                                            ttttAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTGTTTGGGTTTCTAAAATTTTAACTTCGCGTGTTTCGTTGCCGTAAGGGt  <  1:172440/249‑1 (MQ=255)                                                                                                                                                                                            ngTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCa                                                                                                                                                             >  1:124708/2‑78 (MQ=255)                                                                                                                                                                                                              naaTTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAtc                                                                                     >  1:310636/2‑132 (MQ=255)                                                                                                                                                                                                              naaTTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGAATGATCATTGACTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCCCCGTGAATGTCCGCTCTGGCACAGCGCGAAATTTTTTGATCTCCCCCCTGACATCTAACCTTAGTCATGTCACGTTTTTGGGTTTCTGAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAgg    >  1:93585/2‑213 (MQ=255)                                                                                                                                                                                                              cAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAtc                                                                                     <  2:310636/132‑1 (MQ=255)                                                                                                                                                                                                              cAATTCCGTTCCAGGGTTGCTTGTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGTTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTGTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                     <  1:238588/164‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                          GGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGT  >  NC_000913/4510020‑4510439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑