| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,867,430 | G→A | A41V (GCC→GTC) | rpoS ← | RNA polymerase sigma factor RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,867,430 | 0 | G | A | 100.0% | 55.7 / NA | 21 | A41V (GCC→GTC) | rpoS | RNA polymerase sigma factor RpoS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/8); total (13/8) | |||||||||||
CACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCC > NC_000913/2867215‑2867656 | naCGATTGCCATAACGGCGGGCAATTTTTTCCACCCGACCCAAGTTACTCACGATCATCCGCCGGCGAGCGGCGCCACCTCCACGCAGTCCGCGCCGCCCAAAATACACTTCTTCTTCCGCCGTTTACACTGGTGAATAACCAATCTCCCCAAGGGAAAGCTGCGTCGCCCCCAACACCCGCTGTGTGGCTCCCCGCGATAAAAGTTACTCTTAGACCAAATCGTTAACACTGGGTTCCTGTTttacta > 1:92164/2‑249 (MQ=255) cATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCTATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCg < 1:65839/251‑1 (MQ=255) aGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGAGGAGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGAGTTCCTGTTCTACTAAGGCCTTTTCGTCAAACACCTCAACTCCGTTCtcatc > 1:453530/1‑251 (MQ=255) gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGt < 1:475235/193‑1 (MQ=255) cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCt > 1:469347/1‑165 (MQ=255) ncgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCaa > 1:63365/2‑168 (MQ=255) gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCGGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACGAAGGCCTTTTCGTCAAAAACCGCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACtttn < 1:60230/224‑2 (MQ=255) cGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAATGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACg < 1:270147/250‑1 (MQ=255) ggCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACAAGCTGTGTGGCACCCTGCGATAACAGATCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTg > 1:286624/1‑238 (MQ=255) cGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTg > 1:14648/1‑112 (MQ=255) gtggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCAGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTACGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGc > 1:341484/1‑250 (MQ=255) gNNTAACCANNCTCACCAAGGGAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTg < 2:14648/99‑1 (MQ=255) gTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTACCTGCGATAACAGTTCCTCTTCGACCAAAACGTTATCCCTGGGTTAATGTTCTACTAACGCCTTTTCGTCAAAAAACTCAACTCCGTTCTCATCAAATTCCGCATCTTCCTTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCCAGCAAAAGCCTGGTTCCGCCGATTTATCg > 1:495249/1‑251 (MQ=255) gTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGGTCCTCTTCGAACAAATCGTTATCAATGGGGTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGGTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTa > 1:265784/1‑162 (MQ=255) cgTCCAACACACGCTGTGTGGATCCCTGCGATAACAGCTACTCTTCGACCACATCCTTATCACTGGGTTCCTGTTCTACTATGGACTTTTCGTCAAACACCTCAACTCCGTTCTCATAAACTTCCGCATCTTCATTTAAACCATCAACTTTCAGCGAATTCTGACTCATAAGCTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGATCCGCCGATTTATCGCTGCGGCAAATAAc > 1:336534/1‑251 (MQ=255) cAACACACGCTGTGTGGCTCCCTGCGATAATAGTTCCTCTTCGACCAAACGGTTATCCCTGGGTCACTGTTCTACTACGGCCTTTTCGTCAAAACCCt > 1:461967/1‑98 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATNATGNNNNNTcnnnnnnnnnn > 2:306365/1‑144 (MQ=255) gtgtgGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCg < 1:438654/171‑1 (MQ=255) gtgtgGCTCCCTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTAcn < 1:306365/250‑2 (MQ=255) cccTGCGATAACAGTTCCTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGTCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCTGGTTTACGGATTTcccn < 1:150645/251‑2 (MQ=255) ccTCTTCGACCAAATCGTTATCACTGGGTTCCTGTTCTACTCAGGCCTTTTCGTCAAAAACCTCAACTCCGTTATCCTCAAATTCCGCATCTTCa > 1:253046/1‑95 (MQ=255) | CACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCC > NC_000913/2867215‑2867656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |