Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,918,209	C→T	K19K (AAG→AAA)	atpG ←	ATP synthase F1 complex subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,918,209	0	C	T	96.8%	85.1 / NA	31	K19K (AAG→AAA)	atpG	ATP synthase F1 complex subunit gamma
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/17);  minor base G (0/1);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCCAGCAGTTTTTTGAACAGGTTAATGTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAG  >  NC_000913/3917964‑3918449                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gCCAGCAGTTTTTTGAACAGGTTAATGTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTNg                                                                                                                                                                                                                                             <  1:396156/251‑1 (MQ=255)  ccAGCAGTTTTTTGAACAGGTTAATGTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCGGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGc                                                                                                                                                                                                                                            <  1:85360/251‑1 (MQ=255)              tGAACAGGTTAATGTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGaa                                                                                                                                                                                                                                 <  1:186044/250‑2 (MQ=255)                           gTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGAGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACAGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTtac                                                                                                                                                                                                                   >  1:225452/1‑251 (MQ=255)                                              ncACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGcc                                                                                                                                                                                                >  1:51921/2‑251 (MQ=255)                                                         cgACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTgc                                                                                                                                                                                                   <  1:21635/237‑1 (MQ=255)                                                               caccaGGTAGCACACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCtt                                                                                                                                                                                                       >  1:389945/1‑227 (MQ=255)                                                                                         gtcggacTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCCCTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGc                                                                                                                                                                                                                               >  1:69575/7‑177 (MQ=255)                                                                                         cgcgGTCTTCCAGGTATGGGTGCTTATATTCCAGAGTACCGAGTGCAAGGTGACCAATCACATTGCGCATGGTATCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCCTTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTGCTGGACGCTTGCGAGCTTACTACGTATCTCTCTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCGCTAAGGc                                                                                                                                                     <  1:231592/251‑1 (MQ=255)                                                                                                      ggaaGGGGGCTTAAAATCCAGAATACCGTGTGCAAGGTGACCAATCACCTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTGCGCATTTTGGAAGCTGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGGGCTTCTCCTCAATGCCGTTCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                                                                        <  1:491183/249‑1 (MQ=255)                                                                                                        aaGGGTGCTTATATTCCAGATTACCGTGGGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGGTTTTTTGCgtgt                                                                                                                                                                                                                                        >  1:267417/1‑153 (MQ=255)                                                                                                                 tataTTCCAGATTACCGTGTGCAAGGTGTGCAATCTCTTTTCGCATGGTTTCTTCAGAAGTACGGCTGGCCTGCATGCGATCCTGCGATTTACTCATTTTGTAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGa                                                                                                                                                                                                                                  <  1:475639/150‑1 (MQ=255)                                                                                                                      tCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATTCCTTg                                                                                                                                                                     <  1:401636/206‑1 (MQ=255)                                                                                                                              aCCGTGTGCAAGGTGACCAATCTCTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTgn                                                                                                                 <  1:44844/250‑2 (MQ=255)                                                                                                                                 ntgtgCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                                                                   >  1:146782/2‑229 (MQ=255)                                                                                                                                      cAAGGTGACCATTCACTGGGCGCATGGTTTCTGCATAAGGACTGCGGGCCGCCATGCGATCGTGCGATTTACGCATTTGGGAAGCGGCGACCATCTCCATCCCTTTAGTGAGTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTGTTGCGCCGGCCATGAGCGTCTCCTCAATGCTTTGCGGCCTGCGTTTGGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGa                                                                                                        <  1:498382/251‑1 (MQ=255)                                                                                                                                            gACCAATCGCTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGTCCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTATTACTTATCTCTTTGGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATg                                                                                                   <  1:468190/250‑1 (MQ=255)                                                                                                                                                    ncTTTGCGCATGGTTTCTGCATAAGGACCGCTGGCCGCCATGCGATCCTGCGATTTCCGCATTTAGGAAACGGCGACAATATCAATCGCTTTACTGATTTTTTGAGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAg                                                                                                                                 >  1:50207/2‑212 (MQ=255)                                                                                                                                                      tttGCGCCTGGTTTCTGCATAAGGACGGCTGGCCGCCTTGCGATCCTGCGATCTACGCCTTTTGGGAGCGGCGACCATCTCCCTCGGTTTAGTGCTTTTTTGCGCGTTGTGGACGCTTGCGCTCTTACTACGCATCTCTTTTGCGCAGGACATGAGCTTCTCCTCACTACCTTGCGGCCTGCCCTAAGGCAAGCCGCAAGACGTTACCAGGAtt                                                                                                                             >  1:227158/1‑214 (MQ=255)                                                                                                                                                                    cTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAgg                                                                                                                                                      <  1:496055/175‑1 (MQ=255)                                                                                                                                                                                 gCTGGCCGCCATGTGATCCTGCTATTTACGCATGTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATGTTTTGCGTGTTCTGGACGCTGGCGATCTTACTACGTATCTCTTTTGCTCCGGCCAGGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAg                                                                                                                                                  <  1:336305/166‑1 (MQ=255)                                                                                                                                                                                      ccgccATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCt                                                        >  1:267482/1‑251 (MQ=255)                                                                                                                                                                                       cgccATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTAGCGCGTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTGCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTg                                                       <  1:453873/251‑1 (MQ=255)                                                                                                                                                                                        gccATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACg                                                                                                                                                                                                               <  1:431506/98‑1 (MQ=255)                                                                                                                                                                                        gccATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCATTTCAGCTTGCCTACGCTTTCGTCGTTGTAACCACCGGTCTgg                                                      >  1:381051/1‑251 (MQ=255)                                                                                                                                                                                                               cATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                                                                       <  1:290995/195‑1 (MQ=255)                                                                                                                                                                                                                       aaGCGGCGACCATCTCCATCGCTTTAGTGATTTTTTGCGTGCTCTGGACGCCTGCGATCTTACTCCGTCTCTCTTTTGCGCCGGCCATGAGCTCCTCCTCCATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTCCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                           >  1:286490/1‑199 (MQ=255)                                                                                                                                                                                                                          cggcgACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTACTGGACGCTTGCGATCTTATTACGTATCTCTTTTGCGCCGGCCATGCGATTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgc                                                                                                                                                 >  1:362725/1‑126 (MQ=255)                                                                                                                                                                                                                             cgACCATCTCCATCGCTTTAGTGATTTTTTGCGTGTTATGGCCGCTTGCGATCTTACTACGAATCTCTTTTGCGCGGGCCATGAGCTTCTCCTAAATGCCTTGCGGCATGCACTAAGGCACGCCGCCAGACGTTAc                                                                                                                                    >  1:496124/1‑136 (MQ=255)                                                                                                                                                                                                                                 cATCTCCATCGCTTTAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTg                                                                                                                                                                     <  1:376861/99‑1 (MQ=255)                                                                                                                                                                                                                                              ttAGTGATTTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAATCCGGCAGACGTGTCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATTCCTGTCAGCTTGCCTCTGATTTCGTCGTTGTAGCCAGCGTTCTGCTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCagag  <  1:289880/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GCCAGCAGTTTTTTGAACAGGTTAATGTTCAAACCACCGCACAAACCACGGTCGGTCGACACCACCAGGTAGCCCACGCGTTTAACGTCGCGGTCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAG  >  NC_000913/3917964‑3918449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑