| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,030,625 | C→T | G15D (GGC→GAC) | tusE ← | sulfur transfer protein TusE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,030,625 | 0 | C | T | 93.3% | 34.1 / ‑3.5 | 15 | G15D (GGC→GAC) | tusE | sulfur transfer protein TusE |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (7/7); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01 | |||||||||||
GGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCAGTGTTCCGGTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTG > NC_000913/1030381‑1030798 | ggCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCAGTGTTCCGGTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCGTTCTTTGATATAGTCTTCg < 1:139840/250‑1 (MQ=255) cttcGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGACTCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAACACGCACAACTTCCCAGTGTTCCGGCGACAGAGAACTCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATc > 1:332321/1‑196 (MQ=255) ncAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCACAGTGTTCCGGTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTggc > 1:62329/2‑251 (MQ=255) aTTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGATTTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCAGTGTTCCGGTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTc < 1:66088/177‑1 (MQ=255) ncacaAAACGCACCACTTCCCAGTGTTCCGGTGACAGCGGACTCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCgg > 1:44282/2‑251 (MQ=255) naGTGTTCCGGTGACCGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCt > 1:130400/2‑145 (MQ=255) cAGTGTTCCGGTGACAGCGAACTCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgcg > 1:117769/1‑230 (MQ=255) gTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTg > 1:263458/1‑251 (MQ=255) ctaaaTCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATCGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTg > 1:476334/3‑201 (MQ=255) naaNCACNANNGNNAGCNGCTCACTCCACTGGCTGCTTTNNTGGAGATAGNCTTCGGTAGCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTNCCTTAATCANNCTNGCGGTNGNNNANNNAANAACAAAGCCCCGCATAAATNGANNCTATGANAGTGTTAGCGGGTGAGATTAATcgcg < 2:117769/192‑1 (MQ=255) agccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTT‑CTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTTTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGc < 1:425039/193‑1 (MQ=255) ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTAtc < 1:3314/59‑1 (MQ=255) ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTAtc > 2:3314/1‑59 (MQ=255) gcgcACTCCACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGGTTCTATCTCTTTACCTTCGAAGAGCAGCATAATTATTACTACCTTAAGCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgcg < 1:278596/172‑1 (MQ=255) ccACTGGCTGCTTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAAc < 1:10213/107‑1 (MQ=255) ctgctTTCTTTGAGATAGTCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGATGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcagcag < 1:337599/188‑1 (MQ=255) | GGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCAGTGTTCCGGTGACAGCGAAATCCCTTCGTTCTCTGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTG > NC_000913/1030381‑1030798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |