| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,164,123 | C→G | A162G (GCA→GGA) | btuB → | cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,164,123 | 0 | C | G | 94.1% | 35.2 / NA | 17 | A162G (GCA→GGA) | btuB | cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (7/9); minor base T (0/1); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCG > NC_000913/4163960‑4164354 | gCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTAATGCGCTTGTCCAGCGTGTTGAATCTATCCGTGGGAAGAGCTCACCTGTTTATGGTTCCGATGAAATAGGAGGGGAGGTGAATATCATCACGACGCGCGATGAACCAGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAAc > 1:350293/1‑192 (MQ=255) ccGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAt > 1:165396/1‑236 (MQ=255) gTTCCATATTGCGCTTGTCCAGCGTGTTGAATATATCCGCGGGCCGCGCTCCGGTGTTTATGGTTGCGGTGAAATAGGCGGGGCGGTGAATATCAACACGCAGCGCGCTGAACCCGGAAAGGAAAATTAAGGAGGGAGGGGGAGc > 1:491013/1‑145 (MQ=255) tCCATCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGTTGCAATAGGCGGGGTGGTGAATATCATCACGACGCGTGATGAAGCCGGAGCGTAAATTTCAGGAGGGTGGGGAAGCAATAGTTAGCAGAGCTATGATGTCTCTACGCAGCATCAACGGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCGCATGCTGATGGTTATGATGTTGTTGCATATGGTAAt < 1:156339/245‑1 (MQ=255) aaTATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGAtgt < 1:216503/217‑1 (MQ=255) tatCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAAGTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcagca < 1:200371/147‑1 (MQ=255) ccGCTGTTTATGGTTCCGATGTAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCACTACGCAGCAACAACTGGGGGATAAGACACggg < 1:138541/153‑1 (MQ=255) aTGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATTTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACTATGGTTTTTTAAGTAAAAcn < 1:54147/251‑2 (MQ=255) ngTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGCCGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGCGGGGAAGCCATAGTTATCAGAACTATGCTGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGa > 1:54012/2‑207 (MQ=255) gATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTAGGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATgg < 1:119963/251‑1 (MQ=255) gggTGGTGAATATCATCACGACGCGCGCTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGCAGCAATAGTTCTCAGAAATCTGATGTCTCTCCGCAGCAACACCTGGGGGATaa > 1:333398/1‑115 (MQ=255) ttgtgAATATCATCACGACGCTCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAATAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCTGATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTAt < 1:80819/231‑1 (MQ=255) catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGCGGGTGGGGAAGCAATAGTTCTCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACggg > 1:212943/1‑110 (MQ=255) caCGACGCGCGATGAACCCGGAACGGAAATTTCAGTAGGGGGGGGAAGCAATAGTTATCAGAACTTTGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACg < 1:408188/105‑1 (MQ=255) ncgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGAGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGATGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTg > 1:302536/2‑251 (MQ=255) gcgATTAACCCTTAACGGAAATTGCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgggg < 1:466602/86‑1 (MQ=255) cccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGTAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAACCGTTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTgcg < 1:38480/251‑1 (MQ=255) | GCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCG > NC_000913/4163960‑4164354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |