Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,413,728 G→A intergenic (‑263/‑136) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,413,7280GA100.0% 67.5 / NA 23intergenic (‑263/‑136)envR/acrEDNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

ATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAGATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCAC  >  NC_000913/3413561‑3413962
                                                                                                                                                                       |                                                                                                                                                                                                                                          
atTGAGTTGCTATAAATCGGTAAATAAATAATAGATATTATTTACCTAAGATTCATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAGCCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTTGTTATGTGTCTTGAAATGCCTGGAGTCTTAACTATTCCTTACATCGACGAATGATAATTAGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTa                                                                                                                                                          <  1:237369/250‑1 (MQ=255)
        gCTATAACTCGTTAACTAACTAATATATATTATTTACCTAAGATACATTCACTACCTCAATATATATTTCAATTTACGAGGATTTAATTATGCCTCTTTAAACCCGCGTCAAAATAAAAAAGTAGCATATTAATCTTTTTTTGTGTTTATGTGCCTTGAACTGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTACCGCGTTGc                                                                                                                                                 >  1:385827/1‑251 (MQ=255)
                 cGTTAAATAAATAATAGATATTATTTACCTAAGATACATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGGGTTTATGTGCCGTGAAATGCCTGTAGTCATAACTAGTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTgg                                                                                                                                         <  1:321296/250‑1 (MQ=255)
                                          tACCTAAGATACATTCACTACATCAATATATATTTCAATTTACGATGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCGTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGcn                                                                                                                                                                                    <  1:45791/182‑2 (MQ=255)
                                                  aTACATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATANCTANNNNNTannnnnn                                                                                                                                                                                                            >  2:298235/1‑144 (MQ=255)
                                                     cATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGACTATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCAAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAAcg                                                                                                                               >  1:573/1‑224 (MQ=255)
                                                              cATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAAc                                                                                           >  1:216540/1‑251 (MQ=255)
                                                                tCAATATATATTTCAATTTACGNGGTTTTAATNNTGNCTCTGNCNNNCNNNGTNAAAATAAAACAGTAGAATANTANNCTTTTTNTGTGTTTATGTGCCTTGAAATGCCTg                                                                                                                                                                                                                                     <  2:119150/111‑1 (MQ=255)
                                                                tCAATATATATTTCAATTTAAGAGGTTTTAATTCTGCCTCTTTCAACCCGAGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTg                                                                                                                                                                                                                                     >  1:119150/1‑111 (MQ=255)
                                                                             cAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTCTTCATAACTATTCCTTACATCGACGAATGATAATTTg                                                                                                                                                                                              >  1:160133/1‑137 (MQ=255)
                                                                                                tCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTAttn                                                          <  1:298235/250‑2 (MQ=255)
                                                                                                        tttCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCGTTTTTTGTGGTGATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTtttctttc                                                                                                                                                                   <  1:282323/137‑1 (MQ=255)
                                                                                                         ttCAACCCNNGTNNANANAAAACNGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGc                                                                                                                                                                                                                                        >  2:335848/1‑67 (MQ=255)
                                                                                                         ttCAACCCGCGTCAAAAGAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGc                                                                                                                                                                                                                                        <  1:335848/67‑1 (MQ=255)
                                                                                                           cAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCAAGGTTTTTCCTCCTGCCCTCCTTTATTc                                                          >  1:109789/1‑239 (MQ=255)
                                                                                                                     cAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAAc                                                                                                                                                        >  1:5103/1‑135 (MQ=255)
                                                                                                                              aCAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGc                                                                                                                                                 >  1:8077/1‑133 (MQ=255)
                                                                                                                                                 tttttGTGTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGt                                                                                                                                                    <  1:250729/111‑1 (MQ=255)
                                                                                                                                                   tgtgtgTTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAAc                                                                                                                                <  1:284661/127‑1 (MQ=255)
                                                                                                                                                        gtTTATGTGCCTTGAAATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGTTTTTTTTAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCTCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCAc  <  1:416324/250‑1 (MQ=255)
                                                                                                                                                         nttNTGTNCNNTNNAATNCCTGTATTCATAACTATTCCTNNCATCGACGANTGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTNCCTTTTTGGNNAANTAACGNGNNNTNNNTTNTTTGAGGAATAGTAATGACNAANNATGCCANGTTTTTCCTCCTGCCCTCCTTTATTc                                                          <  2:109789/192‑1 (MQ=255)
                                                                                                                                                              gtgCCTTGAAATGCCTTTATTCATAACTATTCCTTACATCGGCGTATGAGAATTTGTAGGATAGCGAGCTGGAATTTTCTTTCTGCGAGTTAACGCGTTGc                                                                                                                                                 <  1:410094/101‑1 (MQ=255)
                                                                                                                                                                gNNTTGAAANNCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGc                                                                                                                                                 <  2:8077/99‑1 (MQ=255)
                                                                                                                                                                       |                                                                                                                                                                                                                                          
ATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATATATTTCAATTTACGAGGTTTTAATTCTGCCTCTTTCAACCCGCGTCAAAATAAAACAGTAGAATATTAATCTTTTTTTGTGTTTATGTGCCTTGAGATGCCTGTATTCATAACTATTCCTTACATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCAC  >  NC_000913/3413561‑3413962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑