Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	513,659	G→A	A362T (GCG→ACG)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	513,659	0	G	A	95.2%	51.0 / ‑6.5	22	A362T (GCG→ACG)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/11);  total (9/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCG  >  NC_000913/513461‑513863                                                                                                                                                                                                       |                                                                                                                                                                                                             aaCCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGACAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGcc                                                                                                                                                          >  1:243877/1‑251 (MQ=255)                                      gCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGc                                                                                                                                                                                              <  1:188634/178‑1 (MQ=255)                                       cAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACg                                                                                                                                                                    >  1:160402/1‑203 (MQ=255)                                                        cGAAGCGAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGcn                                                                                                                                                                                      <  1:56092/168‑2 (MQ=255)                                                                  gAATAAATNCNNNTNNCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATNCTGATGACGGCGGCANTGNATTTNGGCNCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGc                                                                                                                                                                                              >  2:356116/1‑150 (MQ=255)                                                                  gAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGAGGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGc                                                                                                                                                                                              <  1:356116/150‑1 (MQ=255)                                                                          cccTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCGGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCTGTGGCTTTATCTGCAGTCTGTGGTCCCAggg                                                                                  <  1:421458/250‑1 (MQ=255)                                                                                  cggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTATGCTCACTCGCCAGGGTTGCCAGCGCCACCTTTTTGATTTGCTACCTTACGGTGTTTGTGGTGGCGATCCGCCTGCGTCAGGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTAcn                                                                                                                                       <  1:57857/189‑2 (MQ=255)                                                                                  cggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGc                                                                         <  1:409977/251‑1 (MQ=255)                                                                                         gTACCTGGGGCAACATTATTGTCGTGGAGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGCTTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCg                                                                                                                 >  1:159735/1‑204 (MQ=255)                                                                                           aCCTGGGGCAACATTATTTTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGGACGGTGGTGATGTGGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATg                                                                <  1:65940/251‑1 (MQ=255)                                                                                                gggCAACAGTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATGTAGGCTCACTCGCCAGCGTTGCCGGCGCCACCTTTTGGAGGTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTc                                                                                                                                                                       <  1:431654/143‑1 (MQ=255)                                                                                                       attattGGCGTGGTGTTGATTATGCTGATGTCGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGAGTTGCTACCTGACGGTGTGTGTGGTGGCGAGCCGCCTGCGTCATGATATTCACGCCTCGGTGCCGATTCTTATCGTTGGTACGGTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTc                                                    <  1:455855/251‑1 (MQ=255)                                                                                                        ttatgGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTTCCGATTCTTATCGTTGGTAGGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTTCAGTCTGTGGGCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTc                                                    <  1:481331/250‑1 (MQ=255)                                                                                                                               cTGATGACGGCGGCACCGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGAAGGTGTTTgtggt                                                                                                                                                                                                 >  1:227635/1‑86 (MQ=255)                                                                                                                                      cggcggCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTc                     <  1:403879/251‑1 (MQ=255)                                                                                                                                      cggcggCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTc                     >  1:230906/1‑251 (MQ=255)                                                                                                                                           gcACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTcc                                                                                       >  1:183264/1‑180 (MQ=255)                                                                                                                                                         cNCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGATGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCg  >  1:393980/1‑251 (MQ=255)                                                                                                                                                            aCTCGCCAGCGTTGCCAGCGCCACCTGTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCGACGGTCTGTTGTCCCAGGGTAGCCGTGCGTTGATATGGatt                                                            <  1:204956/190‑1 (MQ=255)                                                                                                                                                                  cAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGACGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGt                                                                                                                                  >  1:40694/1‑114 (MQ=255)                                                                                                                                                                                            tttGCGACCTGTCGGTGTGTGTGGTGTGGATCCGCCTGCGTCTTGATTTTCACGCCTCGTTGCCGATTCTTATCGTTTGTTCGTTGTAGATGTTTTTGGTGa                                                                                                                    <  1:176214/102‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                             AACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCATGATATTCACGCCTCGTTGCCGATTCTTATCGTTGGTACGTTGGTGATGTTGTTGGTGATCGTTGGCTTTATCTACAGTCTGTGGTCCCAGGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCG  >  NC_000913/513461‑513863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑