Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,159	G→A	F58F (TTC→TTT)	csrD ←	regulator of CsrB and CsrC decay

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,159	0	G	A	100.0%	37.1 / NA	14	F58F (TTC→TTT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

CCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTCGCGAAACAGATCGCTGGAGCCAACTGGACGATAACTACCATTTCTGGCCAGGGTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGCAAAGTCACAAAAATTGTTAACCCGGTGAGCAGCGTAACAAAGGCCGAAAATTTCGTCGTTAATCTCATCCTTGTGTTAACTCCGATAGTGAGGAAGCGGG  >  NC_000913/3402950‑3403364                                                                                                                                                                                                                  |                                                                                                                                                                                                              cccATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTCGCGAAACAGATCGATGGAGCCAACTGGACGATAACTACCATTTCTGGCCAGGGTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCAc                                                                                                                                                                      >  1:327226/1‑251 (MQ=255)                                                          ncGCTCAGTTCGCGAAACAGATCGCTGGAGCCAACTGGACGATAACTACCATTTCTGGCCAGGGTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAAc                                                                                                              >  1:316768/2‑250 (MQ=255)                                                                                         cAACTGGACGATAACTACCATTTCTGGCCAGGGTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACAGCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAATCTTAGCGAACAGCCCAGCAAAGTCACAAAAATTGTTAACCCg                                                                              <  1:75402/251‑1 (MQ=255)                                                                                            cTGGACGATAACTACCATTTCTGGCCCGGGTATACACCTGCTTATCACAATGCAGCAGGTCTACACGAACGATATCTGCCGCCATCATTAATTCGGTAATTTGTGGCCTTAATAAGCTAAAGTCATTCGACACCAGGCGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGCTGAc                                                                                                                                                     >  1:229643/1‑177 (MQ=255)                                                                                                           cATTTCTGGCCAGGGTATAAACCTGTTTCTCACCATGGAGCAGGTCTACACGCACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTACAGTAATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGCAAAGTCACAAAACTTGTTAACCCGGTGAGCAGCGTAACaa                                                              >  1:471994/1‑249 (MQ=255)                                                                                                                       nggTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGCAAAGTCACAAAAATTg                                                                                      >  1:405053/2‑213 (MQ=255)                                                                                                                                                     ggTCTANNCGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCg                                                                                                                                                                             <  2:14535/96‑1 (MQ=255)                                                                                                                                                     ggTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCg                                                                                                                                                                             >  1:14535/1‑96 (MQ=255)                                                                                                                                                                            gACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAANNCGATGANNa                                                                                                                                                   >  2:5721/1‑99 (MQ=255)                                                                                                                                                                            gACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt                                                                                                                                               <  1:5721/103‑1 (MQ=255)                                                                                                                                                                                            tAATTTGTGGCCTTAATACTCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGTAAAGTCACAAAAATTGTTAACCCGGTGAGCAGCGTAACAGAGGCCTAAAATTTCGTCGTTAATCTCATCCTTGTGTTAACTCCGATAGTGAGGAAGCggg  <  1:69553/228‑1 (MQ=255)                                                                                                                                                                                                     tccTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGCAAAGTCACAAAACTTGTTAAccc                                                                               >  1:391985/2‑142 (MQ=255)                                                                                                                                                                                                     gCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATNNCGTTGTNNa                                                                                                                          >  2:18693/1‑99 (MQ=255)                                                                                                                                                                                                     gCCTTAATACGCTAAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGcc                                                                                                          <  1:18693/115‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                              CCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTCGCGAAACAGATCGCTGGAGCCAACTGGACGATAACTACCATTTCTGGCCAGGGTATAAACCTGTTTATCACCATGGAGCAGGTCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACTTATACTGAATGGCGTTGTAGAAACTTAGCGAACAGCCCAGCAAAGTCACAAAAATTGTTAACCCGGTGAGCAGCGTAACAAAGGCCGAAAATTTCGTCGTTAATCTCATCCTTGTGTTAACTCCGATAGTGAGGAAGCGGG  >  NC_000913/3402950‑3403364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑