Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	55.4 / NA	20	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/8);  total (12/8)

CCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCC  >  NC_000913/3707741‑3708150                                                                                                                                                                                                               |                                                                                                                                                                                                            ccTTTGCAGTAGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGAAGGGAATGACGACCTACTTAACATCAAAGTAACACAGCGGATCCTCAATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCACTACAGACAACCCCGATTTACACCAACGGTGAGACTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGaa                                                                                                                                                                  >  1:369494/1‑250 (MQ=255)                gTCGCTGCCTGGCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCTACCATAAAATGCCCCTCGTGACCGACAGTCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTGACACCAACGGTGAGAATCCATAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgt                                                                                                                                                            <  1:271899/241‑1 (MQ=255)                   gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAAAGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                               >  1:82313/1‑251 (MQ=255)                      gCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGGGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGc                                                                                                                                                          <  1:453819/237‑1 (MQ=255)                                                    ggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                                                                                              >  1:181222/1‑251 (MQ=255)                                                                                         cATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGACTGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAACACTTCAAACAGCTATTTGCAGCAGAGAAACTTGTGCTACTCCACCATGACCAGACCAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCACTTAATGAAAAAAATc                                                                         >  1:24135/1‑251 (MQ=255)                                                                                         cATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                                                                                                                                                           >  1:15241/1‑153 (MQ=255)                                                                                                                           ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATAcc                                       >  1:160912/1‑251 (MQ=255)                                                                                                                            cccTCGGGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCa                                                                                                                                                   <  1:435212/142‑1 (MQ=255)                                                                                                                                           aTCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGAATTTACACCAACGGAGAGAATCCACAAACACAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAACTTTGTGCTACTACAAAATGACCAGAAAAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTACAACGc                       >  1:280768/1‑251 (MQ=255)                                                                                                                                               gNNAACAGANNGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                                                                                                                                                           <  2:15241/99‑1 (MQ=255)                                                                                                                                                 cAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGCGCATCCACCAACAAAGATTCAAAAAACTTCACACAGc                                                                                                                                                                                  >  1:254866/1‑90 (MQ=255)                                                                                                                                                             aaaaCGTCAATACAGCCAGCCGTGAGTTACACCAACGGTGAGAATCCACAAACAAAGAGTAAAAAAACTCCAAACAGGTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAAtn                                                                                                                                  <  1:57532/126‑2 (MQ=255)                                                                                                                                                              aaaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTACAAAACATTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAATAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAggg    >  1:474252/1‑251 (MQ=255)                                                                                                                                                                 cGTCAATACAGCCAACCGGGATTTACACAAACGGTGAGAATCCACAAACAAAGCTTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGgcc  >  1:157670/1‑250 (MQ=255)                                                                                                                                                                  gTCAATACAGCCAACCGGGATTTACACCAACAGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTTGATATCTGAGCGATTAATACCATGAAc                                 <  1:144448/218‑1 (MQ=255)                                                                                                                                                                                    gATTTACACCAACGGTGAGAATCCACAAAAAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGcaca                    >  1:400319/1‑213 (MQ=255)                                                                                                                                                                                                  gTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAATATCTg                                                                       <  1:417215/148‑1 (MQ=255)                                                                                                                                                                                                      naaTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTTTTTGcagcagc                                                                                                                                                                     >  1:93925/2‑49 (MQ=255)                                                                                                                                                                                                      gaATCCACAAACAAAGATTAAAAAAACGTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                                               <  1:460977/88‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                            CCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCC  >  NC_000913/3707741‑3708150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑