Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,568	C→T	P806S (CCG→TCG)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,568	0	C	T	100.0%	46.2 / NA	18	P806S (CCG→TCG)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

GCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGGTT  >  NC_000913/3993356‑3993805                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              gCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAACCGCAAGACGAGAATGGCTTTACTATCTAAATACTCGACGAAAGCAACCGGGATGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGa                                                                                                                                                                                                          >  1:210294/1‑250 (MQ=255)   tttGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTGTCAGATTGTGGAGGTTGATGTTCGTg                                                                                                                                                                                                           <  1:398706/247‑1 (MQ=255)                                         aaCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCTTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCATTTCTATCAGATTGTGAAGGTTTATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgc                                                                                                                                                                 <  1:505349/251‑1 (MQ=255)                                                                                          cGGGTTGAGGTATATCTCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCTGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTc                                                                                                                <  1:357333/251‑1 (MQ=255)                                                                                           gggTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCa                                                                                                                                                    >  1:235197/1‑214 (MQ=255)                                                                                                                     ngcagcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAttt                                                                                                                   >  1:125331/2‑221 (MQ=255)                                                                                                                     ggCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCt                                                                                     >  1:1050/1‑251 (MQ=255)                                                                                                                                                        cTTCTNATCGTNANNGCATGACCGTATTACCTACNGCTCAAGCTTCATCANCTTCNACCTGNCGNa                                                                                                                                                                                                                                           >  2:199730/1‑66 (MQ=255)                                                                                                                                                        cTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTc                                                                                                                                                                                                                                       >  1:209359/1‑70 (MQ=255)                                                                                                                                                        cTTCTACTCGTCATCGCATGACCGTTTGACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTg                                                                                                                                                                                                           <  1:199730/98‑1 (MQ=255)                                                                                                                                                             aCTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCGCAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCan                                                                                                                                             <  1:92147/155‑2 (MQ=255)                                                                                                                                                              cTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATc                                                                                                                                                                                                                                   <  1:295887/68‑1 (MQ=255)                                                                                                                                                                                    aCCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAAc                                                                                                                                                                                                        <  1:5914/73‑1 (MQ=255)                                                                                                                                                                                    aCCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAAc                                                                                                                                                                                                        >  2:5914/1‑73 (MQ=255)                                                                                                                                                                                    aCCTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAGTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGtn                      <  1:97961/251‑2 (MQ=255)                                                                                                                                                                                     ccTACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCATGCCAATCAGGATCACGa                                                                                                                                           >  1:228017/1‑133 (MQ=255)                                                                                                                                                                                       tACGGCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGt                                                                                                                                                                                                    <  1:295454/74‑1 (MQ=255)                                                                                                                                                                                           gCTCAAGCTTCATCAACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTCGATGGTCGTCAACCGGTGATTCCGTTCCGCACACCCTCTCTCGGTAACATGCCGCCTGCCACTCTGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGACAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGCCTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCCGCa                >  1:255605/1‑250 (MQ=255)                                                                                                                                                                                                        caACTTCAACCTGTCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGGtt  >  1:388529/1‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              GCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGGTT  >  NC_000913/3993356‑3993805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑