Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,787	C→T	intergenic (+141/‑63)	argP → / → scpA	DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,787	0	C	T	100.0%	61.7 / NA	24	intergenic (+141/‑63)	argP/scpA	DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/9);  total (15/9)

GTAAAGTCACTGATGCGTTACTCGATTATGGTCACAAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAG  >  NC_000913/3060591‑3060993                                                                                                                                                                                                     |                                                                                                                                                                                                               gTAAAGTCACTGATGCGTTACTCGATTATGGTCACAACGTCCTTCGTCAGGATTAATCCATCAACTACTGCCTGATAGCACCTATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACgg                                                                                                                                                          >  1:267063/1‑251 (MQ=255)   aaaGTCACTGATGCGTTACTCGATTATGGTCACAAAGTCCTTCGTCAGGATTAATCCATAAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTGTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATTCCCTCATTTTGATTGCGTTTTACGGAg                                                                                                                                                        <  1:336993/251‑1 (MQ=255)                          ttATGGTCACAAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAGTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGa                                                                                                                                                         <  1:443868/227‑1 (MQ=255)                               gTCACAAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTGTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATAccc                                                                                                                                                                                <  1:175307/199‑1 (MQ=255)                               gTCACAAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGATGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAGCGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTgg                                                                                                                             >  1:81703/1‑250 (MQ=255)                                 nacaAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTc                                                                                                                                                                              >  1:96723/2‑199 (MQ=255)                                    naaGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGATGCCCTAACTACTTTTTGTAATCCTTGAATCACATAACAACATAGACAAATCTCAGGCGCCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGATCACCGTAGGCGCACATACCCTCAATCTGATTGCGTTTTACGGCGCAAATAATGTCTAACGTGc                                                                                                                                     >  1:57892/2‑237 (MQ=255)                                          cTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGcc                                                                                                                 >  1:155319/1‑251 (MQ=255)                                                                              gCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTAAGGCGGAAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACATACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGAc                                                                             >  1:80251/1‑251 (MQ=255)                                                                                          cGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACGAACCTACGTAAAAAGCGCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTGTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTtcat                                                                 <  1:502337/251‑1 (MQ=255)                                                                                                                                cacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCCGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGc                                  >  1:412292/1‑244 (MQ=255)                                                                                                                                   aaaaTAGACANANCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACNAACCTACGTANAAANNTCACNGNANGCGCAAATACCCTCATTTTGATTGCGTTTNACGGAGCAAATAATGTCTAACGTNNAGNNNTGNNAACAg                                                                                                                       <  2:368224/156‑1 (MQ=255)                                                                                                                                   aaaaTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAGTGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACAGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAg                                                                                                                       >  1:368224/1‑156 (MQ=255)                                                                                                                                           cAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGAAGGAGTGGCAACAGCTTGCCAACAAGGAATCGATCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAAcc                                                           <  1:179704/208‑1 (MQ=255)                                                                                                                                                    ggcggcAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACAGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTACCGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATc       >  1:417706/1‑251 (MQ=255)                                                                                                                                                        gcAAAAAACGACGTCTGACTGCATTTTTTTTGCTGGCGTCTCCCCTACGTCAACAGCTCACCGTAGGCGCAACTACTCCCATTTTGATTGCGTTTTACGGTGCAAATAATGTCTAACGTGCAGGCGTGGCCCAAGCTTGCAAACACGGAATTGAGCCGTCGGGAGAAAACTGTCGAGTCGATGGTTCATCAAAACGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCGGATCTCGATCATCTgg    >  1:458520/1‑250 (MQ=255)                                                                                                                                                         cAAAAAACGACGTCTGAATGCATTTTTTTGGCTGGCTACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAACCCGGGGAAGGGaga                                               <  1:145774/206‑3 (MQ=255)                                                                                                                                                                cgacgTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATCCCCTAATTTTGATTGCGTTTTACGGAGCCAATAATGTCTAACGTGCAGGAGAGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGccctc                                         >  1:81515/1‑202 (MQ=255)                                                                                                                                                                   ngTCTGAATGCATTTTTTGTGCNNGCGACAAACNTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACNGAGCAAATANNGTNTAACGNGNNNGNNNGGNAACAGCTTGCCAACAAGGANTTNNGCCGTCNGGAGAAAACTGTCGACTCGCTGGTtc                                                                   <  2:109330/175‑1 (MQ=255)                                                                                                                                                                   cgTCTNNATNNNTTNNTTTTGCTGGCGACAAACCTACGTNAAAAGCTCACCGTAGGCGCAAATACCCTCNTNTNGATTNNGTTNTACGGAGCAANTAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGANTNGTCGGGAGAAAACTGTCGACTCGCTGGTTCANCAAACCGCGGAAGGGATCGCCATCNAGCCNNTGnannnnnnnnn                     >  2:371046/1‑213 (MQ=255)                                                                                                                                                                   cgTCTGAATGCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTtc                                                                   >  1:109330/1‑176 (MQ=255)                                                                                                                                                                   cgTCTGAATGCATTTTTTTGGCGGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGTCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAg  <  1:371046/241‑1 (MQ=255)                                                                                                                                                                            gCATTTTTTTTGCTGGCGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAAAGAGAAGGAGAGGCAACAGCTTGCCAACAAg                                                                                                           >  1:406888/1‑127 (MQ=255)                                                                                                                                                                                            cGACAAACCTACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACa                                                                                                                        >  1:388293/1‑98 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                               GTAAAGTCACTGATGCGTTACTCGATTATGGTCACAAAGTCCTTCGTCAGGATTAATCCATCAAATAATGCCTGATAGCACATATCAGGCGTTGTCCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAG  >  NC_000913/3060591‑3060993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑