Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,218	G→A	G48G (GGC→GGT)	yphE ←	putative ABC transporter ATP‑binding protein YphE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,218	0	G	A	100.0%	23.2 / NA	11	G48G (GGC→GGT)	yphE	putative ABC transporter ATP‑binding protein YphE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/9);  total (2/9)

CCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTGCCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTGCCTCTGTTGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATC  >  NC_000913/2678046‑2678449                                                                                                                                                                             |                                                                                                                                                                                                                                        ccACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCTCCGTTTTTACCTAACAgcgc                                                                                                                                                                                                               <  1:427022/199‑1 (MQ=255)          gCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCGTCCAGTCGCGTCGCGCCAATCCAGATATCACCGCTATCCGTGCGTTCGCTGCCGGTAATCATTCGAATGAGATTCGATTGACCCGCTCCGTTTTTTCCTAACAGCGCACGATCTTCGCCTTTATTGAg                                                                                                                                                                                          <  1:323428/211‑1 (MQ=255)                           aaCTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGccac                                                                                                                                 <  1:468512/251‑1 (MQ=255)                                               cACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTg                                                                                                             >  1:438729/1‑251 (MQ=255)                                                aCCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTGc                                                                                                            <  1:32057/251‑1 (MQ=255)                                                            gctgCACGGCTAGTCAGCTTAGCTTCGTCCCCACCCAGTCGCCTCTCGCTAATCCATATATCACCGCTATCCGGGCGTTCGCTGACGGTAAGCATTCGAATGAGAGTCGATTTACCcg                                                                                                                                                                                                                                     <  1:328830/115‑1 (MQ=255)                                                                       ngTCAGCGTAGCTTCGAAACCTTCAAGTCGCGTCTCGCCAATCCAGATCTCACCGCTATCCGGGCGTTCGATGTCGGTAAGCATTCGAATGAGCGTCGATTTACCCGCGCCGTTTTTACCTAACCGCGAACGAACTTTGCCTTTATTGAGCGTGAAGTTAACGTTATCCCGCACAACGACGCCGGGATAACGCTTATTTCCTGCCACCCCTTTTGCTACCGGTACTGCCTCTGTTGCCGTGAACATAggg                                                                                      >  1:406252/2‑250 (MQ=255)                                                                                 gCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCGCCGTTTTTACCGAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTGCCTGCCACCTCTTTTGCTACCGGGACTGc                                                                                                            <  1:410200/218‑1 (MQ=255)                                                                                                    gcgTCNNGCNNNTCNNGATATCACCGCTATCCGGGCGTTNGCTGCCGGTAAGCATTCGAATGAGAGTCGNTNTNCCCGNNCCGNTTTTACCTAANAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCNCNACGACGCCGGGATAACGCTTATTTCCTGCCANCACTTTTGCTACCGGGACTGCCTCNGTTGNNGTNannnnnn                                                                                        >  2:381424/1‑213 (MQ=255)                                                                                                          cGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTACCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTAGTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTGCCTCTGTTGCCGTGAACGTAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATc                                                     <  1:433573/248‑1 (MQ=255)                                                                                                                                                 cGGTAAGCATTCTAATGAGAGTCGATTTACCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTGTTGCTACCGGGACTGCCTCTGTTGCCGTGAACATAGGGACCTCGGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGt           <  1:381424/251‑1 (MQ=255)                                                                                                                                                           tCGAATGAGAGTCGATTTACCCGCGCCGTTTTTTCCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGAATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTGCCTCTGTTGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATc  <  1:496484/250‑1 (MQ=255)                                                                                                                                                                             |                                                                                                                                                                                                                                        CCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGAATGAGAGTCGATTTGCCCGCGCCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTATCCAGCGCAACGACGCCGGGATAACGCTTATTTCCTGCCACCACTTTTGCTACCGGGACTGCCTCTGTTGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATC  >  NC_000913/2678046‑2678449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑