Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,763,207	G→A	L112F (CTC→TTC)	abpB ←	putative helicase YfjK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,763,207	0	G	A	100.0%	48.6 / NA	19	L112F (CTC→TTC)	abpB	putative helicase YfjK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

ACATAAACAGCCTGATCGGAATGGCACACTTGGGAACTGTGGTGGATGATCTGATATCGGTCACCGAATCGCTCTTGCAGTCGGCGACGGGTTTCGTCGGCAAGAGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAGCAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGAC  >  NC_000913/2762981‑2763451                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     acaTAAACAGCCTGATCGGAATGGCACACTTGGGAACTGTGGTGGATGATATGATATCGGTCACCCCATCGCTCTTGCCGTCGGCGACGGGTTTCGACGGCACGCGCCCCGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCAt                                                                                                                                                                                                                              >  1:459691/1‑251 (MQ=255)           ccTGATCGGAATGGCACACTTGGGAACTGTGGTGGTTGATCTGATATCGGTCCCCGAATCGCTCTTGCAGTCGGCGACGGGTTTCGTCGGCCAGCGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCCCTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGCACAGTTCGAATCTGTGGAACTGCCTTGTGTGGac                                                                                                                                                                                                                     >  1:183144/1‑249 (MQ=255)                                                              cACCGAATCGCTCTTGCAGTCGGCGACGGGTTTCGTCGGCAAGAGCCACGGTTGGAACAACCAGAACAAGCAGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTc                                                                                                                                                                  >  1:418758/1‑250 (MQ=255)                                                                                           gTTTCGTCGGCAAGAGCCACGGTTGGAACAACCAGAACAAACCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGTCCATACTTGAACAGAGCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTAGAGCTCAAGCACCCGCAGGTCATCCGGTGTCAGGCTGGTGAACTCCttt                                                                                                                                      <  1:438135/249‑1 (MQ=255)                                                                                             ttCGTCGGCAAGAGCCACGGTTGGAACAACCAGAACATGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCAGCCGTTGTCAGTCTGGTGAACTCCGTTTTg                                                                                                                                   <  1:140833/250‑1 (MQ=255)                                                                                                gtcgGCAAGAGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAACCTTGAACAGCTCGAATATGTGGACCTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTg                                                                                                                                   >  1:271361/1‑247 (MQ=255)                                                                                                 tcgGCAAGAGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACANATCNNNNNtg                                                                                                                                                                                                                                         >  2:318243/1‑144 (MQ=255)                                                                                                   ggCAAGAGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGAGTGCCCTTTTGCCCAAGCTTGTAGGTGCACTCAGCGCCACGTTTTGCCCAGACTTGAACAGAGCTAATATGCGGGGCTGCATTGAGTGGAAAACATAACCATCCGAGAATGGc                                                                                                                                                                                               <  1:460771/184‑1 (MQ=255)                                                                                                                                            aaGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATtgtn                                                                                   <  1:318243/251‑2 (MQ=255)                                                                                                                                             aGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTcc                                                                                                                                         <  1:11577/196‑1 (MQ=255)                                                                                                                                             aGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGAACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGaa                                                                                                                                                                                                                                    >  1:246597/1‑105 (MQ=255)                                                                                                                                                   cATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATTGCGTCCGGTATAGCTCAAGCACCCGCAGGTCATCCTGTGTCAGGCTGGTGAACTCCTTTTTGAGAGACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCt                                                                            <  1:274204/251‑1 (MQ=255)                                                                                                                                                        ngAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGCAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGa                                                                        >  1:48645/2‑250 (MQ=255)                                                                                                                                                                            ncACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGCAAACATAACCATCCGAGAATGGCGTc                                                                                                                                                                                            >  1:48837/2‑114 (MQ=255)                                                                                                                                                                             cACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGCACAGATCGAATATGTGGAACTGAATTGAGTGGAAAAc                                                                                                                                                                                                                  >  1:463025/1‑91 (MQ=255)                                                                                                                                                                                    gCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCg                                                                          <  1:473375/220‑1 (MQ=255)                                                                                                                                                                                                    gCACTCAGCACCACGTTTTGACCAGACTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGTTCATCCTGTGTCAGGcg                                                                                                                                                   <  1:145100/131‑2 (MQ=255)                                                                                                                                                                                                                             aCTTGAACAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTGCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCTAGAGCTCGCACGATCAAGTCCCTTCCCAAGTCATACGAAACAGGGTTAGan  <  1:126602/251‑2 (MQ=255)                                                                                                                                                                                                                              cTTGAACAGATCGAATATGAGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCCAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGac  >  1:219230/1‑250 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ACATAAACAGCCTGATCGGAATGGCACACTTGGGAACTGTGGTGGATGATCTGATATCGGTCACCGAATCGCTCTTGCAGTCGGCGACGGGTTTCGTCGGCAAGAGCCACGGTTGGAACAACCAGAACAAGCCGTTTCAAAGTTCCCATGCCGAGCAATGAATCGACGATTGCACTTTTGCCCATGCTTGTAGGTGCACTCAGCACCACGTTTTGACCAGACTTGAGCAGATCGAATATGTGGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGAC  >  NC_000913/2762981‑2763451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑