| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,356,258 | G→A | P285S (CCG→TCG) | sapA ← | putative periplasmic binding protein SapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,356,258 | 0 | G | A | 83.3% | 17.4 / ‑4.9 | 12 | P285S (CCG→TCG) | sapA | putative periplasmic binding protein SapA |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (4/6); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTG > NC_000913/1356081‑1356456 | gTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGGGGGGTTAAATGGCCGATAAGCGACGTTCATCCCCGGACGCAGCTGTTAA‑CGCAAGCACGGGTCGTCACGCCAAAAGGGTTGCTGGGTGGGCGCAGGCCAGG‑CCAGAACAGCGCATTCCCCGGGCAGGAGGTTc > 1:35154/1‑251 (MQ=255) aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCATC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCaggccagg‑cc < 1:474826/219‑1 (MQ=255) cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGATTTGCGGGGGTAAATGCCCGCTAGGGGACGGTCATCCCCGGAAGCAGC‑GGTAAAAGGAACCGAGAGTCGTCACGGCAAATGGCGAGGTGGGTGGGAGCCGGGCAGGGCCGGAA‑GACGCAATTCCCGGTCAGGAGGTTCGACAGACGTCCgggggcgcccgggcctca > 1:9058/1‑234 (MQ=255) attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCTGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACTCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGTATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGTCGCATGCCCCGGTCAGTAGTTGCGACAGACGTCCTGTGCCGCCGGTGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTAc < 1:365568/251‑1 (MQ=255) cGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGGTTAATAGAAGATAAGCGCAGGTCCTCCCCGGACGCAGC‑GATAAACGCAAGCGCGGGTCGTCACGCAAAAAGGGTAGCTGGCTGGCAGCAGGGCAGG‑CCAGAAAGTCGCATTCCCCGGGCAGGGGGTTTGTAAGACGGCCGGGGCCCGCGGAGCCTAAATCCCCCACTACCTGCGGCCTTAACGGGTTAccccggg > 1:424842/1‑246 (MQ=255) gcATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGGATAGCTGGCTGTCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAg < 1:72797/195‑1 (MQ=255) aaTGCCAGCTAGGCGACGTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACg > 1:167379/1‑62 (MQ=255) aaNNNNNGATNGGCGACGTTCACCCCAGGACGCAGC‑GTTAAACGCAAGCGCGAGGCGTCACGCAAAATGGATAGCTGGCGGGCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccg < 2:309419/144‑1 (MQ=255) gCGACGTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGc > 1:354096/1‑205 (MQ=255) gCGACGATCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGAAAAATGGATAGCTGGCTGGCAGCCGGCACGG‑CCCGAACGTAGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCAACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGc > 1:354106/1‑205 (MQ=255) ccAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACt < 1:415565/226‑1 (MQ=255) cAGGACGCAGC‑GTTAAACGCAAGCGCGAGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGACGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGTCTAAGTCCTCCACTGCCTGCTGCATTAACGGTGTACCGCGCCAGAATTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTg < 1:492902/226‑1 (MQ=255) | GTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGG‑CCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTG > NC_000913/1356081‑1356456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |