Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	537,976	C→T	A115V (GCC→GTC)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	537,976	0	C	T	100.0%	39.1 / NA	16	A115V (GCC→GTC)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

GATGGTCGGCGGCTTTTTTATTCTCGGCTTGTCTACCTTTAGTATTATGCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGC  >  NC_000913/537773‑538220                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     gATGGTCGGCGGCTTTTTTATTCTCGGCTTGTCTACCTGTAGTATTATGCTGGCAATGATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGGGCTTCTTACGGTGTACGTGGTGCACTGTTGCCCGGATTATTAAGGGGCGGAGTTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                                                                                                                                        <  1:423162/250‑1 (MQ=255)                 tttATTCTCGGCTTGTCTACCTTTAGTATTATGCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTg                                                                                                                                                                                        <  1:403655/250‑1 (MQ=255)                  ttATTCTCGGCTTGTCTACCTTTAGTAGTATGCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTGTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTgg                                                                                                                                                                                       <  1:361164/250‑1 (MQ=255)                                                 gCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGAGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                                                                                                                                       >  1:470658/1‑251 (MQ=255)                                                                                                           gcGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTctg                                                                                             >  1:204033/1‑251 (MQ=255)                                                                                                                          ggTGTGCCTTTTGCCATGATCCTGCTTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTAGTAAGGGTCGGAATTGTCGCCATCATGTGGTTTGGTGTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTgn                                                                              <  1:43658/251‑2 (MQ=255)                                                                                                                           gtgtGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGCTTATTACGGGGCGGAATTGTCGCCATTATGTGGTTTGGTTTGCAATGTTACGCGGGGTACCTGGCCTGCTTGATTCTGTTTGTCATTTTCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTAATTTCTTAATCTTCTGGCTGGTAAACGTTg                                                                               >  1:495101/1‑249 (MQ=255)                                                                                                                              tgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGGTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAgg                                                                                                                                    <  1:72047/193‑1 (MQ=255)                                                                                                                                    ttGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGGCACTGGCCTGCTTGAGTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTc                                                                                                        <  1:272462/215‑1 (MQ=255)                                                                                                                                                        tcttACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGACTTGTAGCCGTCATGTGgtttggtttg                                                                                                                                                                                                                                >  1:271610/1‑75 (MQ=255)                                                                                                                                                                    cGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGAAATGt                                                                                                                                                                                                                          >  1:430952/1‑69 (MQ=255)                                                                                                                                                                                    ccGGATTATTAAGGGGCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATATGGCCGGGATTTTTAACTCTCGGTGGTGCTTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGc                    >  1:79841/1‑251 (MQ=255)                                                                                                                                                                                                 gggCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGttt                                                    <  1:15583/206‑1 (MQ=255)                                                                                                                                                                                                  ggCGGAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTATCTGGCCTGCTTGATTCTGTTTGGCAAAATc                                                                                                                                                                                >  1:226348/1‑81 (MQ=255)                                                                                                                                                                                                      gAATTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTAAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTcgnc  <  1:395218/251‑1 (MQ=255)                                                                                                                                                                                                        aTTGTCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCa                                  <  1:4342/217‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     GATGGTCGGCGGCTTTTTTATTCTCGGCTTGTCTACCTTTAGTATTATGCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATTACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGC  >  NC_000913/537773‑538220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑