Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,042	G→A	S162L (TCG→TTG)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,042	0	G	A	100.0%	51.0 / NA	17	S162L (TCG→TTG)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/9);  total (8/9)

TGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCC  >  NC_000913/1398852‑1399274                                                                                                                                                                                               |                                                                                                                                                                                                                                         tGCCAGCATGCCGCTTTTCTGGAAACGACCCAGCAGCCGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGACAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGCCAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATct                                                                                                                                                                              >  1:427782/1‑251 (MQ=255)                   cTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGAc                                                                                                                                                                        >  1:115740/1‑239 (MQ=255)                                                            ggCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTa                                                                                                                                                       <  1:209945/215‑1 (MQ=255)                                                                  gANAGTNANNGNNAGCNCCACGAGTCATCGTCAGGCGGNNTTCACGAGGNGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGNCGTTCCTCGNNATNTTTCGNCNNNANNNGCNGGATCATGTCCTGATCGCCNTTNNTTTCACNGCTCATCAGACGCATCATCTGCTGAc                                                                                                                                                                        <  2:115740/192‑1 (MQ=255)                                                                                           cATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGC‑AGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGca                                                                                                                                                                                    >  1:16558/1‑154 (MQ=255)                                                                                                              cGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTc                                                                                                                    <  1:166636/200‑1 (MQ=255)                                                                                                               gAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAcc                                                                                                                                                                                                  >  1:473045/1‑121 (MQ=255)                                                                                                                  gggAGAAGCCGCGTTGGGCAAAACGACGGGACAGGATGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTAAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGAt                                                                        >  1:178933/1‑240 (MQ=255)                                                                                                                                  ggCAANACGCCNGNNCAGGTTGTAGATGAATGCANCCAGACGTTCCTCGGNATTTNTCTTCNacn                                                                                                                                                                                                                                       >  2:213478/1‑64 (MQ=255)                                                                                                                                  ggCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGANTCGNNNnntt                                                                                                                                                        >  2:301217/1‑144 (MQ=255)                                                                                                                                                               aTGCAGCCAGACGTTCCTCGGCATTTTTCGTCAACAACAGCAGGATCATGTCCTGATCGCCTTGGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCg                                                                                                                               <  1:81296/140‑1 (MQ=255)                                                                                                                                                                    nccAGACGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGATCATCAGACGCATCATCTGCTGAc                                                                                                                                                                        >  1:90471/2‑94 (MQ=255)                                                                                                                                                                          cGTTCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTGTCGAACGGGAGTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATgn                                                                      <  1:127497/186‑2 (MQ=255)                                                                                                                                                                             tCCTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcgtcgn   <  1:60637/250‑2 (MQ=255)                                                                                                                                                                              ccTCGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGcn  <  1:301217/250‑2 (MQ=255)                                                                                                                                                                               cgcGGCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGAGGTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTc                                                                                                                                                                <  1:334873/89‑1 (MQ=255)                                                                                                                                                                                   gCATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGAt                                                                                                                                                                                                        >  1:435616/1‑47 (MQ=255)                                                                                                                                                                                    cATTTTTCTTCAACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCgn                                    <  1:405003/210‑2 (MQ=255)                                                                                                                                                                                         gtcttcAACAACAGCAGGATCATGTCCTGATCGCCTTTGATGTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCAGTTGACCGGACAAATCGTCCAGCGTGTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATAGCCGc                                                                  <  1:228276/174‑1 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                                         TGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCC  >  NC_000913/1398852‑1399274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑