| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 431,591 | G→A | R141R (CGC→CGT) | tsx ← | nucleoside‑specific channel‑forming protein Tsx |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 431,591 | 0 | G | A | 92.3% | 26.0 / ‑3.6 | 13 | R141R (CGC→CGT) | tsx | nucleoside‑specific channel‑forming protein Tsx |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (6/6); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
CGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAA‑CGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTAC > NC_000913/431368‑431834 | cGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAA‑CGGTAACCGTCCCACGCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTTGTATTTCGCATAGTCGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTATTACGACCCATGTc < 1:486412/250‑1 (MQ=255) gTAATCGGCACAAAGTATTTAATTTTGAAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATGACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACg < 1:191177/230‑1 (MQ=255) cacaAAGTATTTAATTTTGAAA‑CGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTcgcg > 1:170956/1‑202 (MQ=255) acaAAGTATTTAATTTTGAAA‑CGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCtt > 1:277540/1‑251 (MQ=255) aTAGTTCTGCTACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACg < 1:385649/164‑1 (MQ=255) ntGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGGGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCCGTATTGGt > 1:311640/2‑177 (MQ=255) cANNNNNAGGNCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGt < 2:311640/144‑1 (MQ=255) cAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCGTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGATCCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAATAATACCGGCGCATCCGCATAAc < 1:261046/251‑1 (MQ=255) gtgtCNCTATCNGNNACCAGACCCATGTACCAGGNGCTCTGACGACCATCNTTATNACGACNCANgn > 2:195601/1‑66 (MQ=255) gtgtCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTGAGTACTACCCATGTCGTAAAGGTAGGTGTTCGCGAAGGACCGCTCTTTGAACGGACTGAATCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga < 1:195601/148‑1 (MQ=255) gtggcGATATCGGTTCCCAGACCCATGTACCAGGTGCTCTGACGACCATCTTTAGTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTa < 1:237340/88‑1 (MQ=255) cccATGTACCAGGTGCTCTGACGACCATCTTTCTTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGtt > 1:75085/1‑167 (MQ=255) tGCTCTGACGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTAGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGCCAGTATTCGTCAGCTTGTCGATGGAGAAACGTGGCTCGATTTTCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCTTAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTAc > 1:252637/1‑251 (MQ=255) | CGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAA‑CGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTAC > NC_000913/431368‑431834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |