Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,661	A→T	intergenic (+503/‑108)	nth → / → dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,661	0	A	T	100.0%	55.5 / NA	20	intergenic (+503/‑108)	nth/dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/7);  total (13/7)

CAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGG  >  NC_000913/1712424‑1712901                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAACAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCg                                                                                                                                                                                                                                     >  1:289366/1‑251 (MQ=255)      cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTCCCCGGAAGGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAATGGCAAGATAGGGTACATTACCTGCCGTTTcn                                                                                                                                                                                                                                <  1:129247/251‑2 (MQ=255)                                  naCTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGt                                                                                                                                                                                                    >  1:311598/2‑251 (MQ=255)                                          cACTAGTAAAAACGCTAAATAGCATAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCGGGATAACATTTCCCGGAATGGTTTAAGTCCCCGCCTCAGTTAGATGTAACAGATGATTACAAAGGACTTGTCTGAAAGGGCAAGATAGGGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGtttttt                                                                                                                                                                                               <  1:429886/248‑1 (MQ=255)                                                                                tAAGCCATCCCCCCCCTCTCAGTG‑AATGAAAAAAAGCTGCCGTTAATTTTTTTGAAAAATTTAACGCTGAATAACATTTCCCGGAATGGTTGATTCCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCTTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGt                                                                                                                                                       <  1:382656/250‑1 (MQ=255)                                                                                   gCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTACCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTAc                                                                                                                                                                                                                                           >  1:501629/1‑163 (MQ=255)                                                                                           cccccTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATg                                                                                                                                                             <  1:143691/233‑1 (MQ=255)                                                                                            ctccTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGt                                                                                                                                           >  1:339863/3‑250 (MQ=255)                                                                                             cccTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTa                                                                                                                                          >  1:331227/1‑250 (MQ=255)                                                                                                     gtgcagtaAAAAAA‑TCTGCCTTTACGTTTTTTGAAAAATTTAACGCTGGATAATATTTCCCGTAATGGTTGAATTCCCCGCCGAAGTGATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTTCCGTTTCCCCTCCCTCTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTcc                                                                                                                                 <  1:335222/251‑1 (MQ=255)                                                                                                                           ttACGTTTTTTGAAAAATTTAACTCTGGATACCATTTCCCGGAAGGGTTAAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAAc                                                                                                             <  1:24945/250‑1 (MQ=255)                                                                                                                                      gAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACACAGGACTTGTCTGAACGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCAc                                                                                                                                                                          >  1:88788/1‑178 (MQ=255)                                                                                                                                                     ggATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGATATATGTATAAGATTATTACAAAGGACTTGTATGAAAGTGACAGATAGTGTACCTTAACTGCCGTTTACCCTCCCTCTATAACAATTGCGCGTATGTTTTTTATAAATAACGCGAGAAAGCACCCCCGTTCATATGGGATGTAAAAAAag                                                                                                                                               >  1:328159/1‑190 (MQ=255)                                                                                                                                                       aTAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTATATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAaac                                                                                <  1:213895/251‑1 (MQ=255)                                                                                                                                                                         gTTGAATTCCCCGACTCAGTTATATGTAACAGATTATTACAACGGACTTGTCTGCACGTGCACGATAGAGTACATTACCTGACGTTTCCCCTCCCACTATAAAAATTGCGCGTATGTTTTTta                                                                                                                                                                                              >  1:65584/1‑123 (MQ=255)                                                                                                                                                                                  cccGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACa                                                                                                                                                                                          >  1:34735/1‑118 (MQ=255)                                                                                                                                                                                     gCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGACAGCGTCAGTTTGAACGCTTTCAaac                                                                                >  1:320679/1‑221 (MQ=255)                                                                                                                                                                                                                                     cAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCCAAAACCAACTGAAAGCGTCAGTTTGAACGCtt                                                                                      >  1:71203/1‑167 (MQ=255)                                                                                                                                                                                                                                     cAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATgg  >  1:144139/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTAtc                                                              >  1:3185/1‑189 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 CAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAA‑TCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGG  >  NC_000913/1712424‑1712901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑