Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	401,991	C→T	A82V (GCC→GTC)	phoA →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	401,991	0	C	T	100.0%	62.6 / NA	23	A82V (GCC→GTC)	phoA	alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

TACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATG  >  NC_000913/401784‑402217                                                                                                                                                                                                                |                                                                                                                                                                                                                                   tACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGTGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAgg                                                                                                                                                                                          <  1:344741/250‑1 (MQ=255) tACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGTATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAgg                                                                                                                                                                                          <  1:474936/250‑1 (MQ=255)           cccTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCct                                                                                                                                                                               >  1:401643/1‑251 (MQ=255)                      aaGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGTGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgc                                                                                                                                                                         <  1:78325/246‑1 (MQ=255)                                          ccTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgcttacc                                                                                                                                                                    <  1:187947/231‑1 (MQ=255)                                                                  cAGGGCGATATTACTGCACCCGTCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAAcc                                                                                                                        >  1:30121/1‑251 (MQ=255)                                                                    gggCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgc                                                                                                                                                                         <  1:107457/200‑1 (MQ=255)                                                                                                 nccGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCAcc                                                                                                           >  1:307622/2‑233 (MQ=255)                                                                                                         aCGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTg                                                                                 >  1:231586/1‑251 (MQ=255)                                                                                                          cGGGTGAGCAGACTGCCGCGCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATGTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGGCGAAGGTGCGGGCGGCGTTTTGAAGGGTGTAGATGGCTTGGCGCTTACCGGGCAATGCACTCACTATGCACTGAATAAAAAAACCGGCAAACCGGACTACGTCACCg                                                                                                          <  1:438216/225‑1 (MQ=255)                                                                                                            ggTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATCAACCTGCACAACATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGc                                                                                                                                                                >  1:178865/1‑169 (MQ=255)                                                                                                             gTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAACCTATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTAAGTCACCGACTCGGATGCATCAGCAACCGCCTGGTc                                                                              >  1:343967/1‑250 (MQ=255)                                                                                                                           gCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAACATCTTTTTTTGCTGCTTGGCGCTGGGATGGGGGACTCGGAACTTACTGTCCCACGTAATTATTCCGAAGGTCCGGGCGGCTTTTTTTCAGGTATTGATGCCTTACCGCTTACCGGGCACTACACTCACTCTGCGCTgcctc                                                                                                                                        >  1:75639/1‑174 (MQ=255)                                                                                                                                     aTTCTCTTAGCGATAAACCTGAAAAAAATATTATTTTGCTGATTGGCGAGGGGAGGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTGCCGCTTACCGGGCAATACACTCCTTATGCGCTGAATAAAAAAACCGTCAAACCGGACTACGTCGGCGTCTCGGCTGGATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACgg                                                      <  1:87371/250‑1 (MQ=255)                                                                                                                                           ttAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATgc                                                                                                                                                <  1:386397/154‑1 (MQ=255)                                                                                                                                              gCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGCCTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGc                                            >  1:327051/1‑251 (MQ=255)                                                                                                                                               cNATAAACCTGCAAACAATATTATTTTACTGCTTGGCGATGGGATGGGGGACTCGGAACTTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCg                                           >  1:393368/1‑251 (MQ=255)                                                                                                                                                  tAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGACGGGGGACTCGGAAATTACTATCGCAAGAAATTATGCCGAAGGTGAGGAAGGCTTTTTTAAAGGAACAGATGCCTTACCGCTTACCCGGCAATACACTCAATATGCGCTGAATAAAACAACCGGCAAACCGGACTACCTCACCGCCTCGGCTGCATc                                                                                             >  1:445492/1‑198 (MQ=255)                                                                                                                                                                                  ncGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTg        >  1:318466/2‑251 (MQ=255)                                                                                                                                                                                  gCGATGGGATGGGGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGAGGCCTTACCGCTTACCGGGGAATACACCCACTATGCGCTGAAGAAAAAAACCGGGAAACCGGACTACTTCACCGAGTCGGCTTCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAAcn                                                       <  1:101566/204‑2 (MQ=255)                                                                                                                                                                                        ggatgggGGACTCGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCACCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATg  >  1:449298/1‑251 (MQ=255)                                                                                                                                                                                          atnnnNNACTNGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCAcc                                                                                                           <  2:307622/144‑1 (MQ=255)                                                                                                                                                                                                    cGGAAATTACTGTCGCACGTAATTATGCCGAAGGTGCGggcggc                                                                                                                                                                                                     <  1:338808/44‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                                   TACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATG  >  NC_000913/401784‑402217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑