Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,537	G→A	W548* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,537	0	G	A	100.0%	46.9 / NA	17	W548* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

GGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGC  >  NC_000913/631305‑631765                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     ggTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGATGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGGTCTGTGCGTACTGGCGTGGGTGTACGTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGc                                                                                                                                                                                                                     <  1:491397/250‑1 (MQ=255)                                                                                   gCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctc                                                                                                                                                                                   >  1:110926/1‑202 (MQ=255)                                                                                               nggcngnntnnCTCNGGGCCTGAAGCGGACCGATTCNNTGCCTGCTANCCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGNCGTGGTCGANNCGNTGGGCNGNNNTNNNACNCTGTGACCGCTGTTTGGTANTGNNAACCAGNTGCTGGCAGGGATGGCGCTGATGctc                                                                                                                                                                                   <  2:110926/182‑1 (MQ=255)                                                                                                                                   aCTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCCTTAACACTCTGCGACCGCTGGTTGGTATTGCCAAc                                                                                                                                                                                                                 >  2:316807/1‑124 (MQ=255)                                                                                                                                   aCTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGCGACCGCTGTTTGGTATTGCCaan                                                                                                                                                                                                                 <  1:316807/124‑2 (MQ=255)                                                                                                                                      gcctgcTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGAGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGgcgc                                                                                                                               >  1:375278/1‑203 (MQ=255)                                                                                                                                          gcTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCACTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGAt                                                                                                      >  1:7574/1‑224 (MQ=255)                                                                                                                                           ctagccTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTg                                                                                                   <  1:22542/222‑1 (MQ=255)                                                                                                                                                     ggCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGcc                                                                >  1:264500/1‑251 (MQ=255)                                                                                                                                                                       aCTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGGTGGCGCTGAAGGTCTGTGGCGTGGGGTTGNTccnnnnnggnn                                                                                                                                                       >  2:306880/1‑136 (MQ=37)                                                                                                                                                                       aCTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTcn                                                                                                                                                                <  1:306880/137‑2 (MQ=255)                                                                                                                                                                                  gCTACTTCCTCCATCAGGGCGTTGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGGCAACGTTACGCCTGGGTGGCGCTGTTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCg                                                 <  1:86584/237‑1 (MQ=255)                                                                                                                                                                                     aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCCGTGACAGCTGATTGGTATTGCACCCCCGATGCTGGCAGGGATGGCACTGATGCTCTGTGCCGTAGTGTTGTTCAAGATGCAACGTCAACGCTAc                                                                                                                                            >  1:84478/1‑143 (MQ=255)                                                                                                                                                                                                      gTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGTGATGGCGCTGATGCTCTGTGCCGTGGTGTGGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGt                                                                                                                           <  1:81899/143‑1 (MQ=255)                                                                                                                                                                                                                   tGGGCGGCATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATCCTCTGTGCCGTGGTGTTGTTCAAGATGAACCGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGc  >  1:471464/1‑251 (MQ=255)                                                                                                                                                                                                                       cggcATTAACACTCTGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGCTGCTCTGTGCCGTGgtgt                                                                                                                                                                      >  1:69195/1‑83 (MQ=255)                                                                                                                                                                                                                                  ctctGTGACCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGTGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCTCGGTTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGtttn                                                                   <  1:56526/171‑2 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     GGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGC  >  NC_000913/631305‑631765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑