| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,706,152 | G→A | G518G (GGC→GGT) | dppA ← | dipeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,706,152 | 0 | G | A | 92.0% | 59.4 / ‑5.7 | 25 | G518G (GGC→GGT) | dppA | dipeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/14); minor base T (0/2); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAGCCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTTTGTAGCACCATTTTGAGTAGTTGGAGCCTTGTTCAGAGGCGGCGCAGCTGAACAGGGTGGCGAAGAAGTTATCCGGATCCCC > NC_000913/3705910‑3706394 | gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTGGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGATACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAAc < 1:259984/251‑1 (MQ=255) ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATc < 1:176911/249‑1 (MQ=255) gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGa < 1:186837/250‑1 (MQ=255) cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGc < 1:117596/251‑1 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATcan < 1:317545/251‑2 (MQ=255) gCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGCTAAGGGGACCGCATCGGGCATTTTTGCCTTTTGCAGGAGACATGTGTGGCTTTTAATTATTGGATAGGGACGGTTTAGCGGTGATGTTTGCCTGATGGATCAAGCACATAACCTTTAACTTCTTACCGTACCgg > 1:238191/1‑171 (MQ=255) cATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACTTTTTCGAAGTGAGGTGGGCCTAGAGGATCAACCACTTATCCTTTAACTTCTTGACGGACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTg < 1:455067/191‑1 (MQ=255) aTTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGGTCAAACACGGTGGAGGGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATtgtg < 1:277877/251‑1 (MQ=255) ncAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCACCCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGAGGAGTGAGCGATGATCAg > 1:96605/2‑185 (MQ=255) gAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGgtcgtc > 1:218027/1‑251 (MQ=255) cGTAGGTCTGATAAGCGGAGCGCATCATGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACGTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGc < 1:350363/250‑1 (MQ=255) cGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATAGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGc < 1:350341/250‑1 (MQ=255) aGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTATTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGACTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCa > 1:251082/1‑136 (MQ=255) gCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTGCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATcacc < 1:156437/172‑1 (MQ=255) ttgcctttgccATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGtttn < 1:311414/250‑2 (MQ=255) ntttgccATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCCACCACATAACCTTTAActtc > 1:104865/2‑95 (MQ=255) gCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATTAACCACATAACCTTTAACTTATTTACGTACCGGTTCAAACACGGAGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGTTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTTTGTAGCACCATTTTGAGTAGTTGGAGcc > 1:502005/1‑251 (MQ=255) taatgatgcGATAGAGACGTTTTCGAAGTGATGTTTGCCTAGTGGATCAACCACATATCCTTTAAGTTCTTTACGTACCGGTTCAAACACGGGGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATc < 1:282899/125‑1 (MQ=255) tAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCAACACCTGCGCTTGTTTGTACAGTTCAAcgc > 1:26292/1‑153 (MQ=255) gTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAACCTTTAGCTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTTCCGGAGCCTGATCGTGCATcaccacn < 1:146738/121‑2 (MQ=255) tGCCTAATGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGc < 1:245516/95‑1 (MQ=255) aTGGATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTTTGTAGCACCATTTTGAGTAGTTGGAGCCTTGTTCAGAGGCGGCGCAGCTGAACAGGGTGGCGAAGAAGTTATcc < 1:78723/251‑1 (MQ=255) ggATCAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGGGCGCGATGATCCGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTCCAGTTCAACGCGTTTCTTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAGCGGTTTGTAGCACCATTTTGAGTAGTTGGAGCCTTGTTCAGAGGCGGCGCAGCTGCCCAGGGTGGCGAAGACGTTATCCgg > 1:249411/1‑251 (MQ=255) cAACCACATAACCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTGTGTAGCACCAttttn < 1:130724/186‑2 (MQ=255) aCCACATAACCTTTAACTTCTTTACGTCCCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTTTGTAGCACCATTTTGAGTAGTTGGAGCCTTGTTCAGAGGCGGCGCAGCTGAACAGGGTGGCGAAGAAGTTATCCGGATcccc > 1:164420/1‑251 (MQ=255) | GAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCACATAGCCTTTAACTTCTTTACGTACCGGTTCAAACACGGTGGAGTGAGCGATGATCAGTGCCGGAGCCTGATCGTGCATCACCACCTGCGCTTGTTTGTACAGTTCAACGCGTTTATTGTGGTCGTCGGTAGCACGCGCCGGTTGAATCAGATCTTCAAACGGTTTGTAGCACCATTTTGAGTAGTTGGAGCCTTGTTCAGAGGCGGCGCAGCTGAACAGGGTGGCGAAGAAGTTATCCGGATCCCC > NC_000913/3705910‑3706394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |