| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,329,672 | C→T | A114V (GCC→GTC) | sohB → | S49 peptidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,329,672 | 0 | C | T | 95.2% | 55.3 / ‑6.7 | 21 | A114V (GCC→GTC) | sohB | S49 peptidase family protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (12/8); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACAT‑GATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGC > NC_000913/1329490‑1329918 | gaTGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGc < 1:18923/242‑1 (MQ=255) gcgcTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGCAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTg > 1:73881/1‑171 (MQ=255) gcTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCtg > 1:136933/1‑251 (MQ=255) cAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatggtgcatg < 1:120618/250‑1 (MQ=255) aaaCAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTGAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCAGGAATTGAACTCGCTACGTGAAGAGATAACGGCTGTACGCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatggtgca < 1:498964/242‑1 (MQ=255) ncgcAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAACGCGCAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGCAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGc > 1:298379/2‑196 (MQ=255) gcAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCCGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAAc < 1:160555/251‑1 (MQ=255) gaaAAAGCATAAGCAAGAAGCGAACGCAGCAAAAGCGAAAGCCAAACTGAGCTCGGAGCCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAAATAACGGCTGTACTCGCAGCATTCAAACCGCCGGATCAGGTTGTGCTCCGTCTGGAAAGACCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCaac > 1:321222/1‑249 (MQ=255) aagaagCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGTCATCATGGGCGTCCATGAAGTGAACTCGCTACTTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCTCAGTATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACgg < 1:326369/214‑1 (MQ=255) gaagCGAAAGCAGAAAACGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTtgt > 1:384626/1‑170 (MQ=255) gcaAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAAc > 1:191444/1‑251 (MQ=255) naaaGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGAAGTCCATGAAGTGAACTCGCTACGTGAAGAGATACCGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACgg > 1:97596/2‑251 (MQ=255) nnnnnnaaNNNNNCGANGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGc < 2:298379/144‑1 (MQ=255) cAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGCCGTCCATGAAGTGAACTAGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAgcggc > 1:274227/1‑251 (MQ=255) aGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTgn < 1:53099/58‑2 (MQ=255) cgcgTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGGTACAT‑GATGGGCtg > 1:400563/1‑251 (MQ=255) cTGGGTGCTGGATTTTAAAGGCAGCATGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGc < 1:64057/107‑1 (MQ=255) tGGGTGCTGGATTTTAAAGGCAGAATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATACCGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGGTCTAT‑TATTGCCTGTGTggg > 1:179778/1‑250 (MQ=255) gATTTTAACGGCAGCATGGACGTCCATGCAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGGTAAat‑gaagtaccgtgtggggggggaaa > 1:427561/1‑229 (MQ=255) gcagcaTGGACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatggtgc > 1:27273/1‑117 (MQ=255) gACGTCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGATGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGCTACATCTATGGC‑TGTGTGGCGGGCaaaaatatttcttccccgtttgg > 1:214540/1‑232 (MQ=255) | GATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACAT‑GATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGC > NC_000913/1329490‑1329918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |