Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	37.1 / NA	12	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/5);  total (7/5)

TACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGG  >  NC_000913/1306533‑1306953                                                                                                                                                                                                            |                                                                                                                                                                                                                          naCATCCTTACACCAGGGCATTGATGTCGGCAGTCACCATACCTGATCCAGATCTGGAGAAGAACAAAAACATCCCGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTCTGTTTTCCGTACCCGTTGCCCGATTCCCGGTCCCGAGTGCGCCAAAACACGACCTGTTCTGGAGGGGAGTTTCAGACACGACGTTTCTTGCCTg                                                                                                                                                                                                             >  1:47574/2‑218 (MQ=255)                                                                                    gAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTTTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgcgn                                                                                         <  1:97566/251‑2 (MQ=255)                                                                                                 cttCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGTTCGCCGGTCCAGAGTGCGCCACAGCACGTCCTGCTCTCGAGGGGAGTTTCAGACACGCCATTTATTGCCTGAAAGTCGATCCGCTTTAACAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTACAAGCGATTATTAATTCCCGCCAGATGATATGGCACAGTTTGTGATCtttt                                                                                               >  1:461637/3‑232 (MQ=255)                                                                                                     nccGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                                       >  1:309117/2‑204 (MQ=255)                                                                                                     nccGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGATTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGccc                                                                                                                                                              >  1:47031/2‑165 (MQ=255)                                                                                                                             tgtTTTCCGTCCCCGTTGCCCGTTTGCCGGTCCAGAGTGCGCCAAACCACGTCGTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATcc                                                                                                                                                                                                  <  1:372458/105‑1 (MQ=255)                                                                                                                             tgtTTNNCGNNNCCNNTGCCCGATTGCCGGTCCAGAGTGNGCCAAAACACGTCCTGTTCTGGAGGGGAGNTNCNGACANNCCGNTTCTTGCCTGNAAGTCGATcc                                                                                                                                                                                                  >  2:372458/1‑105 (MQ=255)                                                                                                                                                 cGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCTGCCCTTTTTCAAGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCTAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCa                             >  1:8009/1‑250 (MQ=255)                                                                                                                                                            ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTATTGCCTGAAAGTCGATACGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCg                 >  1:325188/1‑251 (MQ=255)                                                                                                                                                              ngagTGCNCCAAAACACGTCCTGTTCTNGAGGGGAGTNTCAGANNNGNCGTTNCTGGCCTg                                                                                                                                                                                                             <  2:47574/60‑1 (MQ=255)                                                                                                                                                               nnggnnnnnaaanCACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                                       <  2:309117/137‑1 (MQ=255)                                                                                                                                                                   gcgnCAAAACACGTCCTGTTCTGNAGGGGAGTTNCAGACNNNCNGTTTNTTCCCTGa                                                                                                                                                                                                            <  2:56829/57‑1 (MQ=255)                                                                                                                                                                           acacGTCCTGTTATGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGCAAACCGATCCGCTTTAAAAACAATACCGGCTGAAAATAGTCACCCCTTTCTCACCCTAAAAGCGAATATTTATTCCCGCCAGATGATATGGCAACGTTTGTGATCTTTTTCGCGCAATAACAGAATACGCGCAAAAACAACGTTGATTTCACCATCTTCACTGTTAGCCAGACCACTCACCACTTCGTCAAAAAAGTACggg  >  1:350634/1‑251 (MQ=255)                                                                                                                                                                                    nttCTGGNGGGGAGTTTCAGACACGCCNTTTCTTGCCNGAAAGNNNANCCGCNTTTAAAAc                                                                                                                                                                                       <  2:52697/60‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                          TACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGG  >  NC_000913/1306533‑1306953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑