Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,881,160	G→A	A328V (GCT→GTT)	casC ←	type I‑E CRISPR system Cascade subunit CasC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,881,160	0	G	A	100.0%	53.3 / NA	20	A328V (GCT→GTT)	casC	type I‑E CRISPR system Cascade subunit CasC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)

CGGAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAG  >  NC_000913/2880956‑2881371                                                                                                                                                                                                             |                                                                                                                                                                                                                    cGGAAATCTTCCGGTATGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGAt                                                                                                                                                                        <  1:219608/250‑1 (MQ=255)  ggAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCTGTTCAGACCATATCCATTGGCAAcg                                                                                                                                                                                        <  1:412056/233‑1 (MQ=255)                                                 ggCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGATCAGTAATTGGGTCTACATCAGATAAGCTTAATTGCGCAGCAACTCCGTTCAGACCATATCCATTTGCAACGCGATCCCAAGATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGc                                                                                                                       <  1:84260/251‑1 (MQ=255)                                                            gCCCAGCAAGNCNCAAGATCAAATAAGATCTCATGTTCACGTCGCGCCATTATTACGAACCNAGGATTTTAANTGTNNTAAANTNGNCATTTGTTTAACTTGAGCAGTAATTGGGTNTACATCAGATAAGCTGAATTGCGNNGCNNNTCNNTTCAg                                                                                                                                                                                                           <  2:369682/156‑1 (MQ=255)                                                            gCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGTCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGCATTGCGCAGCAACTCCGTTCAg                                                                                                                                                                                                           >  1:369682/1‑156 (MQ=255)                                                                                                       tCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGAAGAAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACAATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTAc                                                                 >  1:282765/1‑251 (MQ=255)                                                                                                                      aCCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATGTTTCGCTTTAACCGCTTTGTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGtt                                                  <  1:462074/251‑1 (MQ=255)                                                                                                                           ggATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAg                                                                                                            <  1:409813/188‑1 (MQ=255)                                                                                                                             aTTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTGGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATATAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCg                                           <  1:222149/251‑1 (MQ=255)                                                                                                                                ttAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGNc                                         <  1:396128/250‑1 (MQ=255)                                                                                                                                           aaaGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACg                                <  1:175110/248‑1 (MQ=255)                                                                                                                                                   cATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAAcgcg                                                                                                                                                                                      <  1:348856/90‑1 (MQ=255)                                                                                                                                                   cATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAAAGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATc                                                                                                                                      >  1:259504/1‑138 (MQ=255)                                                                                                                                                   cATTTGTTNANNNTNNGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACNCCGTTCAGACCATATNCANTGGCNAcgng                                                                                                                                                                                      >  2:348856/1‑90 (MQ=255)                                                                                                                                                           tAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTGCAAAAGCATTTGCCATAGAAAGTGGCAGATCGGAGAAATTAACCATGACCAGATCCGCAGGGGTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGan              <  1:56158/250‑2 (MQ=255)                                                                                                                                                                     aGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAACTCCGTTCCGACCATTTACATTGCCAACGCGCTCCCATTCTTGATTAACCGCCTGTATAGACGGTTGCAAATAGC‑ATCTTTCGGTTTACACGCTTTTTCAAAAGCATCTGCCATAGACAGTGGCATATccg                                                                                 >  1:251803/1‑170 (MQ=255)                                                                                                                                                                              ggcTACATCAGATAAGCTGAATTGCGCAGCAACTCCTTACAGACCATATCCATTAGCAACGCGATCTCAATATTGATTAAACGCCTGTATCGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGACAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAg                                    >  1:481005/3‑209 (MQ=255)                                                                                                                                                                                        gATAAGCTGAATTGCGCAGCAACTCCGTTCAGACCATATCAATTGGCAACGAGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAAACGc                                                                                                                       >  1:324153/1‑116 (MQ=255)                                                                                                                                                                                             gCTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATACCAATATTGATTAAAAGCCTGtata                                                                                                                                                          >  1:189687/1‑76 (MQ=255)                                                                                                                                                                                              cTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTTATTAAACGCCTGTATAGACAGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAAGTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAg  <  1:502975/227‑1 (MQ=255)                                                                                                                                                                                              cTGAATTGCGCAGCAACTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACAGTTGCAAAAAGCCATCGTTCGCCTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAAGTAACCATTACCATATCCGCAGTGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAg  <  1:502992/227‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                    CGGAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAACGCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAG  >  NC_000913/2880956‑2881371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑