Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	509,329	G→A	A684V (GCC→GTC)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	509,329	0	G	A	95.0%	48.8 / NA	20	A684V (GCC→GTC)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/11);  minor base C (1/0);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGCGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGTTGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCAT  >  NC_000913/509089‑509554                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   agcgCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCAtt                                                                                                                                                                                                                          >  1:187513/1‑251 (MQ=255)        ccGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCggc                                                                                                                                                                                                                    <  1:473680/250‑2 (MQ=255)                      aGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACGGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCTCCATTGCCACCTGACGTCCTTCACTTGGCAGATGTTTGATCTCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGGTCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATcccc                                                                                                                                                                                                     <  1:380511/251‑1 (MQ=255)                                                        ccatggcatcctcccTGCCGCCACCCACCGCCGTGCCCTCATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCTCCCACCATTGCCTCCTGACGTCCTTCTCTTTGCATATGTTTGATCGCTTCGGCCTTACCGTCCGGCAGCTCCCCTGCGATCACCTCATCAATCCCTGTTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTACCTCCGGTCATCATCACCAGACGATATCCCGCTTTATGc                                                                                                                                                                   <  1:458978/237‑1 (MQ=255)                                                                                        aTGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGAGGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACCAGATCCCGCTTTATGCAGGCGTTGCAg                                                                                                                                                        <  1:152736/230‑1 (MQ=255)                                                                                                                       cgtcgtTAATTCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTGTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGGGGTTGGGTTATCCCCGGTCAACATCACCAGACGATAGCCCGCTTGATGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATCGCGTACTGCCAGCAGGGCTAcc                                                                                                   <  1:489938/251‑1 (MQ=255)                                                                                                                                    gTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCCGCACCCCGGCGATCTCCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATAACCAGACGATATCCCGCTTTATGCCGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATCGCGTACTGCCAGCCGGGCTACCGCTTTCCCGTCa                                                                                       >  1:252246/1‑250 (MQ=255)                                                                                                                                         ccaccaTTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGCTCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCCGACGATATCCCGCTTTATGAAGGCGTTGCAGCGCCGCCCCGCTCTCACTAC‑ACAACGGATCGCGt                                                                                                                     >  1:163284/1‑215 (MQ=255)                                                                                                                                          caccaTTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCAGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGGGGTTGGGTTATCCCCGGTCACCATCACCCGCCGATATCCCGCTTTCTGCCGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATcg                                                                                                                        >  1:292811/1‑211 (MQ=255)                                                                                                                                                                  tcTTTTCAGATGTTTGAGCCCTTCGGCTTTACCTTCCGGCAGCACCCCGTCGATCACCTCACCACTCCCTGCTTCTTTGACGATCGCATGGGCGGTGGTTGGGTTATCCCCGGTCAACAtaa                                                                                                                                                                                          <  1:458421/121‑3 (MQ=255)                                                                                                                                                                        cAGATGTTTGATCGCTTCGGCTGTACCGTCCGGCAGCACCCCGGCGATCACCGCATCAAGCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGCAgg                                                                                                                                                                <  1:194166/142‑1 (MQ=255)                                                                                                                                                                        cAGATGTGTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATCGCGTACTGnc                                                                                                               <  1:394125/190‑1 (MQ=255)                                                                                                                                                                                            cTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTCGCCGATCGCATTGGCCGTGGCTGGGTTATCCCCGGTAAACATCAACAGACGATTTTCCGATTTCTTCCCGCGTTGCCGCGCCGCCCCGCTACCCCTCC‑GCAACGGATCGCGTACTGCCAGCCGGGCTACCGCTTTCCCGCCAACCGCCAGCACCACAGGCGTTGCCCCCTGCGATGCCTGAGCAGTAATTTCCGCTTct                              >  1:290445/1‑250 (MQ=255)                                                                                                                                                                                                aCCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGGTATCCCCGGta                                                                                                                                                                                                 >  1:222299/1‑84 (MQ=255)                                                                                                                                                                                                      nggCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTTTGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATc                                                                                                                         >  1:93069/2‑150 (MQ=255)                                                                                                                                                                                                      cGGCAGCACCGCGGCAATCACCTCATCAATCCCTGCTTCGTTGACGATCGCATTTGCGGTAGTTGGGTTATCCCCTGGCAACATCACCGGACGATAGCCCGCGTTATGCATGTGTTCCAGCGCCGCCACGCTATCACTAC‑GCAACGGATcgc                                                                                                                       <  1:323285/152‑1 (MQ=255)                                                                                                                                                                                                           gcaCCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATc                                                                                                                                                                              <  1:10620/93‑1 (MQ=255)                                                                                                                                                                                                           gcaCCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGGTGGGTTATCCCCGGTCAACATCACCCGACGNNATc                                                                                                                                                                              >  2:10620/1‑93 (MQ=255)                                                                                                                                                                                                                      accACCTCATCAACCCCTGCTTCTTTGACGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATTGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGTTGCCCCTTGCGATGCCTGAGCAGTAATTTCCGc                                  <  1:116832/219‑1 (MQ=255)                                                                                                                                                                                                                        naCCTCATCAATCCCTGCTTCTTTGACGATCGCATTGGCGGTGGGTGGGGTATCCCCGGGCAACATCACCAGGCGATATCCCGCTTTATGCCGGCGTTGCAGCGCCGCCACGCTATCACTACCGCA‑CGGATCGCGTACTGCCAGCAGGGATACCGCTTTCCCGTCAACCGCCCGCAGAACCGGCGTTGCCCCTTGCGATGCCTGAGCAGTAATTTTCGATTCGGTAGCTTTGGTAACCACCTGTTGCCCAt  >  1:43668/2‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   AGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGCGATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGCAGGCGTTGCAGCGCCGCCACGCTATCACTAC‑GCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGTTGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCAT  >  NC_000913/509089‑509554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑