Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,136,053	G→A	V164M (GTG→ATG)	flgH →	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,136,053	0	G	A	100.0%	55.1 / NA	21	V164M (GTG→ATG)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

GCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAT  >  NC_000913/1135835‑1136283                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       nctcctcTGCGAATGCCAGCCGTGCCGGTAAAACTAATTTTAGCTTTGATACTGTGCCGCGCTCTTTGCAGGGGCTGTTTGGTAACGCTCGTGACGATGTCGAAGCCTCCGGTGGTCACACGTTCAACGGAAAGGGCGGGGCCAATGCCCGCACTACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTCCTGGTCAACGGCAACCTGCATGTGATGGGTGACAAACAGATTGCCATTAATCCGGGt                                                                                                                                                                                                        >  1:46061/2‑251 (MQ=255)                        gACGGTAAAACTAAGTTTGGCGTGGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCGCGTTCCTATGTCGAAGCCGCCGGTGGTAACACGTTCATCGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCTCGTGGACGGTGACGGATGATCAGGTACTCGTCAACGGCAACCGGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTGCCGAATTTATGCGCTTCTCTgg                                                                                                                                                                                 <  1:199603/251‑1 (MQ=255)                          cGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCg                                                                                                                                                                               <  1:398334/251‑1 (MQ=255)                                                      ggcgatcattgaatGCAGGGGCTGTTGGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGGCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgcag                                                                                                                                                   <  1:109918/238‑1 (MQ=255)                                                           ncgcTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGCAAGGGCGGGGCCAATGCCAGCACTACCTTTAGCGGCCCGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAAc                                                                                                                                                                                                                            >  1:53702/2‑173 (MQ=255)                                                                         ggCTGTGTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTAcn                                                                                                                                                                                                     <  1:43465/182‑2 (MQ=255)                                                                                                        aGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAg                                                                                                 <  1:196592/251‑1 (MQ=255)                                                                                                        aGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGACAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGaaaa                                                                                                                                                                                                                              >  1:448433/1‑126 (MQ=255)                                                                                                         gCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCTGATTACCAGGTACTGGTCAACGGAAACCTGCATGTGATGGGTGGAAAACAGATTGCCATTACTCAGGGTACCGAATTTATTCGCTTATCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCACTACCGTACCGTCTACTCAGGGCGCGGATGCGCGCATTGAATACGTAgg                                                                                                >  1:380527/1‑251 (MQ=255)                                                                                                                                           gggCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCTGCAGCAATACCGTACCGTCTACTCAGGTGGc                                                                                                                        <  1:336266/193‑1 (MQ=255)                                                                                                                                             gCCAATGCCAGCAATACCGGGAGCGGCACGTTGGCGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTTCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATGTATTCGCTTCTCTGGCGTGGTTAATCCACGCATTATCAGCGGCAGCAATACCGTACCGTCTGCTCAGGTGGCGGATGCGAGCATTGAATACGTAGGCAAGGGCTACATTAACGAAgcg                                                                          <  1:479944/237‑1 (MQ=255)                                                                                                                                                             ccTTTAGCNGNNNGNNGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGNGATGGGTGAAAAACANATNGCTANTAANCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACg                                                                                                                                                                  >  2:353180/1‑133 (MQ=255)                                                                                                                                                             ccTTTAGCGGCACTTTGACGGTGACTGTTGACCATGTACTGGGCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACg                                                                                                                                                                  <  1:353180/133‑1 (MQ=255)                                                                                                                                                                                 gtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCCGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGGAGCAGCGTTTCTTCCTTACCCTGTc                                 >  1:380741/1‑242 (MQ=255)                                                                                                                                                                                    acggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGTTGGCGGATGCGCGCATTGAATACTTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAAcc                                     <  1:339896/235‑1 (MQ=255)                                                                                                                                                                                             cAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATGAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTggag                                                                                                                       <  1:469137/144‑3 (MQ=255)                                                                                                                                                                                                     ggTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGAGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATg                          >  1:447023/1‑229 (MQ=255)                                                                                                                                                                                                      gTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTc                                                                                                                               <  1:141036/127‑1 (MQ=255)                                                                                                                                                                                                      gTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCAATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCCGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTaa    >  1:210990/1‑250 (MQ=255)                                                                                                                                                                                                          aCGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCTCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATgggn                                                              <  1:95645/188‑2 (MQ=255)                                                                                                                                                                                                                    gCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAt  >  1:425348/1‑238 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       GCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAT  >  NC_000913/1135835‑1136283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑