Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,035	G→A	A246T (GCG→ACG)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,035	0	G	A	83.3%	21.4 / NA	12	A246T (GCG→ACG)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/4);  minor base C (2/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACAGCCCGACTGAACTGATCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGC  >  NC_000913/2194795‑2195222                                                                                                                                                                                                                                                 |                                                                                                                                                                                            tACAGCCCGACTGAACTGATCGAGACGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGAGTGATTCTGAACACTTCTTCTTTGATCTGCCCTATTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGCCAAATAAAATGCAGGAGTGGGTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTAATTCGGTTTTGAAATTCCGAAACCGCCGGGCa                                                                                                                                                                                    >  1:482861/1‑250 (MQ=255)                         ccGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCCGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGc                                                                                                                                                           >  1:438394/1‑251 (MQ=255)                                                     cGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAg                                                                                                                               <  1:189292/251‑1 (MQ=255)                                                                                                                 aggtaTGGACACGCAGAGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAAAAGTGGGATATCTCCCGCGACGCCCCGTACTTCGGTTTTGAAATGCCGAACACGCCGGGCAAATATTTCTACGTCGGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATGCTGGAAGAAATACTcn                                                                   <  1:121534/247‑2 (MQ=255)                                                                                                                         aCCCGCAGCGGTGCGTTGCAGGAGCAGGGGGCAAATAACATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGGTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCgcggc                                                                                                          >  1:86710/1‑204 (MQ=255)                                                                                                                          cccGCAGCGGTGCGTTGCAGGCGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCAccgccg                                                            >  1:398870/1‑249 (MQ=255)                                                                                                                                      cGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCAGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCAccg                                                               >  1:134204/1‑234 (MQ=255)                                                                                                                                                        cAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCtgt                                                                                                                        >  1:135869/1‑159 (MQ=255)                                                                                                                                                               aaTGCAGGCGTGGTTTGAATCTGGCCNGCAACANNGGNATATCTCCCGCGACGCCCCNTANNNCNNNTNAGAAANTCNNAACCCGCCGNGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCNAGACTCTGGNNGACAAGCGCGGCGGCAGCGGANGATNNGNNGNAtcncgn                                                                               >  2:113963/1‑188 (MQ=255)                                                                                                                                                                                     ggCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAAGATTTCTACGTCGGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCGGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGTAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGc  <  1:341736/248‑1 (MQ=255)                                                                                                                                                                                                                    ccccTTACTTCNGTTTTGAAATTCCGAACACGCCGGGCAANTATTTCTACGTNTGGCTNNNc                                                                                                                                                             >  2:69727/1‑62 (MQ=255)                                                                                                                                                                                                                    ccccTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGAc                                                                                                                                                             <  1:69727/62‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                            TACAGCCCGACTGAACTGATCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGC  >  NC_000913/2194795‑2195222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑