Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,037,203	C→T	V302I (GTC→ATC)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,037,203	0	C	T	100.0%	49.5 / NA	20	V302I (GTC→ATC)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

AATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCAC  >  NC_000913/3036991‑3037395                                                                                                                                                                                                                     |                                                                                                                                                                                                 aaTACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGGCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTg                                                                                                                                                            <  1:455412/251‑1 (MQ=255)    aCCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGgca                                                                                                                                                                         >  1:9813/1‑235 (MQ=255)                                 gATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTTCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTTGCCCCAGCGCAAAAnc                                                                                                                                            <  1:394303/235‑1 (MQ=255)                                 gATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                                            >  1:16880/1‑251 (MQ=255)                                            aGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGc                                                                                                                 <  1:134710/251‑1 (MQ=255)                                                                   gCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCGTCAAGTTGCATTCCTTGTTCGATCTCTTTTCGCGTGTGGTTTAGCGCACCGTGGTCATTTGCCAGCACGCGCGTTTCGCCGATGTTGTCGCGCAACAACAGCGCAACCCCGATGGACATATCGGCCAGTCGTCCGGCAGCATTGAGACGTGTCCCCAgcgc                                                                                                                                                  <  1:263557/195‑1 (MQ=255)                                                                           ccAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCCCGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAg                                                                                                                                                     >  1:72012/1‑184 (MQ=255)                                                                                            gTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCCAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACtt                                                                 >  1:376266/1‑251 (MQ=255)                                                                                                    ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTtg                                                                                                                    <  1:144262/192‑1 (MQ=255)                                                                                                                    tGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAtac                                          <  1:481803/250‑3 (MQ=255)                                                                                                                                         nnnnnnnncnctnngAGTNTATTGGCCAGCCCGCGCGCTTCGCNGATGTTGTCGCACATCAACAGCGCCACACCGNTNGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCANAACCTAAATCNCTGNNGGCGNGNTNTTGTGCATCACGGTTTGCCACTTCAAGCANCGCTTTAATCCCCGGACGGCACTNNCCNNNTCNNATGCg                                                  <  2:376266/207‑1 (MQ=255)                                                                                                                                           gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACACCAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAANCGCNnnnngc                                                                                                                             >  2:310562/1‑144 (MQ=255)                                                                                                                                           gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgn                                                       <  1:310562/214‑2 (MQ=255)                                                                                                                                                        aGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGAACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAgcgaaa                                                                                                                                                >  1:277822/1‑109 (MQ=255)                                                                                                                                                           tNNTTTGCCNNCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGc                                                                                                                                                          <  2:9289/99‑1 (MQ=255)                                                                                                                                                             aTTTGNCAGCANGNNAGCTTCGCCGATGTTGTCGNACAACAACAGCGCCANACCGNTGGACntan                                                                                                                                                                                          >  2:188725/1‑64 (MQ=255)                                                                                                                                                             aTTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTGTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCac  <  1:188725/249‑1 (MQ=255)                                                                                                                                                                 gCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCAGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa                            >  1:367556/1‑219 (MQ=255)                                                                                                                                                                             gcTTCGCCGATGTTGTCGTACAACAAAACCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCACAACCTCAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGcc   >  1:168419/1‑231 (MQ=255)                                                                                                                                                                               tgcGCCGATGTGGTCGCACAACAACAGCGCCACACCGATGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGGGGCCCCAGCTCAAAACCTAAATCGCTGGCGGCGAGTTGTTGTGCATCAc                                                                                                            <  1:278201/123‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                 AATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCAGTTTCTCGCACAGGGTCAGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCAC  >  NC_000913/3036991‑3037395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑