Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,642,547	G→A	P196S (CCG→TCG)	rsmJ ←	16S rRNA m(2)G1516 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,642,547	0	G	A	100.0%	68.2 / NA	26	P196S (CCG→TCG)	rsmJ	16S rRNA m(2)G1516 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

GACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGGTCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAGGCGTGAATTAACTGCAACCGCTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGG  >  NC_000913/3642313‑3642765                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            naCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑g                                                                                                                                                                                                             >  1:103614/2‑250 (MQ=255) gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAgatccgatcc                                                                                                                                                                                                                          >  1:35840/1‑238 (MQ=255)  aCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTCCCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑Ga                                                                                                                                                                                                            >  1:248025/1‑250 (MQ=255)                                                      aCCCTGTGTTAGTTTTACCCCGGCGTACCTGCATAGATAGCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGTCGACATTCGCCAGCGGTGGCGCGTAGGCCGGGCGCTTTACCACCACTCGTGTGGTCTCCAACAATCGTGCATGCTCCAGTAATCCATCGGCATCAATAGCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAAc                                                                                                                                                         <  1:375916/249‑1 (MQ=255)                                                                cagagctcactggaggacatGCATAGATATCAAAGAGATGCCCTTTAGTGCCGACATCGGTTTGCGTTGCGACATTTGCCAGCGGCGGTGCGTAGTCCGTGCGTTTTACCGCCCCGCGGTTGTGCGCCAGCAAGCTTGCCGGCCCAAGTAATCCTGCGGCTTCAAGATCCTATCCCACCATCCTCTTGATA‑CCACGAAGCTCTTTGTTCACCCTCTCGCTTTCCTGCTTATGCGGGAACATCGGGTCgag                                                                                                                                              <  1:364088/231‑1 (MQ=255)                                                                       aactanngtnnntgnngcgATATCAACGCGGTGCCCTTTNGTGACTACCGCGTTTTGCGTGGCGACATTNGNCNGCGGNNGCGNGTGGTCCGGGNGCTTTACCACCACGCGCTTGGTCGCCAGCAAGCGTGCAGGATGCTGTANTNCATCGGCATCACGATCCGATCCCACCAGCGGNA‑GGAAGACAAGCTTCGGTTTTTTCNCTAGNNCGNtn                                                                                                                                                                           >  2:364088/19‑213 (MQ=255)                                                                           ggCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGCCTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTACTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTg                                                                                                                                  >  1:443953/1‑251 (MQ=255)                                                                                             tCAAAGCGATGCCCTTTAGTGACGACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGGCCGTGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCAGCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCgtg                                                                                                                                                            <  1:66226/207‑3 (MQ=255)                                                                                                         ccTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTCGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGcc                                                                                                      >  1:261169/1‑249 (MQ=255)                                                                                                                               gcgtggcgACATTCGCCAGCGGTGGCGCGGAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCCGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACCTCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCCGGCTGGAGGCGTGAAtt                                                                                 >  1:344066/1‑248 (MQ=255)                                                                                                                                              ncAGCGGNNGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAgatccgatcc                                                                                                                                                                                                                          <  2:35840/96‑1 (MQ=255)                                                                                                                                              ccNNNNNTGGNGCGTAGTCCGGGCGCTTTACCGCCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCttt                                                                                                                                                                          <  2:312622/144‑1 (MQ=255)                                                                                                                                                           gTAGTCCGGGCGCTTTACCACCACGCGATTGGTCGCCAGAACGCGTGCCGGATCCAGTAATCCAAAGGCATCACGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTa                                                                                                                                                                  >  1:322806/1‑139 (MQ=255)                                                                                                                                                              gTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAgc                                                                                                                                                                                 <  1:451051/121‑1 (MQ=255)                                                                                                                                                                            ccaccaCGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCCGTAATCCATCGGCATCAAGATCCGATCACACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATAtcagtcag                                                                                                          >  1:447453/1‑178 (MQ=255)                                                                                                                                                                            ccaccaCGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAACCCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAGGCGTGAAtt                                                                                 >  1:75282/1‑203 (MQ=255)                                                                                                                                                                             caccacGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCCGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGc                                                                                                                                                               >  1:222498/1‑124 (MQ=255)                                                                                                                                                                                cacGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCccacn                                                                                                                                                                                                                      <  1:315309/67‑2 (MQ=255)                                                                                                                                                                                cacGCGTTTGGTCGCCAGCAAGCGTGCAGGCACCAGTAATCCATCGGCATCAAGATCCGATCccacc                                                                                                                                                                                                                      >  2:315309/1‑67 (MQ=255)                                                                                                                                                                                 acGCGTTTGGTCGCAAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GCAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCg                                                                                                                                                              >  1:172197/1‑121 (MQ=255)                                                                                                                                                                                              gtgtgctctgccgatcgtCCAGTAATCCATCGGCATCAAGCGCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTCCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTAGAGATAACCCACCTGCGGTCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAGTCGTGAATTAACTTCAACCGCTCCGTCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCcgtcg               <  1:382036/234‑1 (MQ=255)                                                                                                                                                                                                      cGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGAAAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGACAGGCTGGAGGCGTGAATTAACTGCAACCGCCCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAg       <  1:187485/251‑1 (MQ=255)                                                                                                                                                                                                           aGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAng                                                                                         <  1:393609/164‑1 (MQ=255)                                                                                                                                                                                                           aGGCTCCAGTAATCCATCGGCATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAGGCGTGAATTAACTGCAACCGCTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCgg  <  1:338569/251‑1 (MQ=255)                                                                                                                                                                                                                               cATCAAGCTNCNNTCCCACCNNCGACT‑GNNAGANAAGCATCTCTTTTTTCACTAGCGCGCTTTTCTNCTTANg                                                                                                                                                                >  2:415153/1‑73 (MQ=255)                                                                                                                                                                                                                               cATCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCGTTTGTCACTAGCGCGCTTTTCTGCTTATg                                                                                                                                                                <  1:415153/73‑1 (MQ=255)                                                                                                                                                                                                                                 tCAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGACCATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGAACTATAAGTCAGCGCCGTCAGGCTGGAGGCGTGAATTAACTGCCACCGCTCCTGCAACCAGCCGCCGATTTccgcatccgcatcac                                >  1:480265/1‑196 (MQ=255)                                                                                                                                                                                                                                  cAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAgcgc                                                                                                                                                                               <  2:22113/55‑1 (MQ=255)                                                                                                                                                                                                                                  cAAGATCCGATCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAgcgc                                                                                                                                                                               >  1:22113/1‑55 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            GACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAGCGTGCAGGCTCCAGTAATCCATCGGCATCAAGATCCGGTCCCACCAGCGACT‑GAAAGACACGCATCTCTTTTTTCACTAGCGCGCTTTTCTGCTTATGCGGGAACATCGGGTCGAGATAAACCACCTGCGGGCGCGGGGTAATATCAGTCAGCGCCGTCAGGCTGGAGGCGTGAATTAACTGCAACCGCTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGG  >  NC_000913/3642313‑3642765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑