Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,743,057	G→A	D62D (GAC→GAT)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,743,057	0	G	A	100.0%	48.0 / NA	18	D62D (GAC→GAT)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/11);  total (7/11)

CAGAATATATTTCATCAACTGACTATCCTGGACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCC  >  NC_000913/1742838‑1743234                                                                                                                                                                                                                            |                                                                                                                                                                                  cAGAATATATTTCATCAACTGACTATCCTGGACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCg                                                                                                                                                    <  1:118557/251‑1 (MQ=255)        tatTTCATCAACTGACTATCCTGGACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCTTAACGTTTCTTTCATCAGATCAAAACTGAc                                                                                                                                                                        <  1:422970/224‑1 (MQ=255)             catcaACTGACTATCCTGGACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACGTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAgg                                                                                                                                         <  1:453118/250‑1 (MQ=255)                                                                             gCCACTTCAGCAGTTGTCNTNATATGTCCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCNCACGCTCAACNTTTNNCCAANCNCNCTCTTGTAAATCGCCAAGATTGGTAATGCNTAACGTTTCTTTCATCAGATCAANNCTNNNATNNTTGcc                                                                                                                                                               <  2:364172/164‑1 (MQ=255)                                                                             gCCACTTCAGCAGTGGTCATAATATGACCTGACATTACGTGTCCGCCAATTTCATCACTGAATTTCGCCGAACGCTCAACGTTTACACAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGcc                                                                                                                                                               >  1:364172/1‑164 (MQ=255)                                                                                            gTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTGTTAAATCGCCAAGATTGGTAATGCGTAACGTTGCTTGCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAg                                                                                                                                          <  1:240401/170‑1 (MQ=255)                                                                                                        ccTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGgcagc                                                                                                                                     <  1:145407/163‑1 (MQ=255)                                                                                                               ttAAGTGTCCGCCAAGTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAAGGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAAGTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGTCCGTCCAGCAGTTGGTCGGGTAACTCCACCACATGc                                                              <  1:165296/227‑1 (MQ=255)                                                                                                                       ccGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGacac                              >  1:215884/1‑251 (MQ=255)                                                                                                                                                  gcACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCCTCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATg                                                                                                                           >  1:232223/1‑131 (MQ=255)                                                                                                                                                   cACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCg                                                                                                                                                    >  1:30102/1‑105 (MQ=255)                                                                                                                                                           ncGTTTANNCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCg                                                                                                                                                    <  2:30102/96‑1 (MQ=255)                                                                                                                                                                  cccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTNTTTNATCANATNAAAACTGACATTGTTNCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACNNANNNANCAGTTTcc                                                                                                     <  2:355508/137‑1 (MQ=255)                                                                                                                                                                  cccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGAGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGCAGAACCGGTTTcc                                                                                                     >  1:355508/1‑137 (MQ=255)                                                                                                                                                                    cAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATcccc  >  1:14044/1‑234 (MQ=255)                                                                                                                                                                         ccccACTTTTAAATCACCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGATCAAAACGGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAGCCTTTATGCGCCACGTAAGCACCTTTTTCCAGGCCGTCCAGCAGGTGGTCGGGTAACTccacca                                                                   <  1:485377/164‑1 (MQ=255)                                                                                                                                                                                   aaaTCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCCGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCggg                                                                              >  1:177664/1‑143 (MQ=255)                                                                                                                                                                                                                  ctttcATCAGATCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCAtg                                                                                      <  1:175501/104‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                  CAGAATATATTTCATCAACTGACTATCCTGGACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCC  >  NC_000913/1742838‑1743234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑