Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	428,681	C→T	L345L (CTC→CTT)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,681	0	C	T	90.5%	54.3 / NA	21	L345L (CTC→CTT)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/10);  minor base G (0/2);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTTGTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGC  >  NC_000913/428506‑428904                                                                                                                                                                                |                                                                                                                                                                                                                                aCGTTGACCAGGCCGCTGCGTGATCCGGTCGCGTACTGGGCGACTCTGAAGTGAAACAGACTCGCGAAGGTCAGCCAGTTGTGCTGTACAAACGCGTAATGCTGACCGGTGACCATAGCTCCGACTCCACTTCCAGCCAGGCCGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGCTAACATCATGTCTAACTTCACGAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg                                                                                                                                                      <  1:221229/251‑1 (MQ=255)        ncAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTTGTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATgg                                                                                                                                                                    >  1:101525/2‑230 (MQ=255)           ggCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGACGTGAAACAGAACCGCGAAGGTCAGCCAGTTGTGCTGTACAAACGCGTAATTCTGCCCGGTGACCATATCACCGACTACACTTCCAGCCAGGACGAATACACCCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACaa                                                                                                                                              >  1:344606/1‑249 (MQ=255)                                                                 cgAAGGTCAGCAAGTTGTGCTGTACAAACGCGTAATCCTGACCGGTGACAATATCACCGACTCCACTACCAGTCAGGACGAATACAACCAACCGCAGGTTAACATCACGCTGGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAgg                                                                                      <  1:14926/251‑1 (MQ=255)                                                                     ggTCAGCCAGTTGTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTACACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTaagaaag                                                                                                                             >  1:447859/1‑208 (MQ=255)                                                                                    cTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGCCTCCACTTACAGACAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAAAATCg                                                                                                                                                                                >  1:473329/1‑142 (MQ=255)                                                                                                        gACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCACCCGCAGGATAACATCTCGCTTAATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCCACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCACCATCCAGTCTCGTCTGGGTAAc                                                >  1:500750/1‑250 (MQ=255)                                                                                                                       ccccGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCgg                                                                                                                                     <  1:2824/148‑1 (MQ=255)                                                                                                                                   ngncanccagGNCGAANACAACCAACCGCAGGNTAACATCTCGCTGGATAGCNNTNGAGGTNAcatc                                                                                                                                                                                                            <  2:202860/64‑1 (MQ=255)                                                                                                                                     ncagccagGACGNATACAACCAACCGCAGGCTAACATCTCGNTTGATAGCGCt                                                                                                                                                                                                                        <  2:70092/52‑1 (MQ=255)                                                                                                                                     ccagccagGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCt                                                                                                                                                                                                                        >  1:70092/1‑53 (MQ=255)                                                                                                                                      cagccagGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATTTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCg                                                                                                            <  1:321037/160‑1 (MQ=255)                                                                                                                                          cagGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTgg                                                                                                                                                     <  1:22148/115‑1 (MQ=255)                                                                                                                                          cagGACGAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACAGCATTTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAAGCCTGTTTGTTGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGc                                                                                                             <  1:273696/155‑1 (MQ=255)                                                                                                                                             gACGAATACAACCAACCGCAGGTTAACATCTAGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCANNCCGATGNNa                                                                                                                                                                  >  2:32869/1‑99 (MQ=255)                                                                                                                                                gAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACNNATGGCann                                                                                                                                                                >  2:24174/1‑96 (MQ=255)                                                                                                                                                gAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAAc                                                                                                                                                                <  1:24174/98‑1 (MQ=255)                                                                                                                                                gAATACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTg                                                      <  1:431473/204‑1 (MQ=255)                                                                                                                                                   tACAACCAACCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACGGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGtn                                                                                                   <  1:404215/156‑2 (MQ=255)                                                                                                                                                     caaccaacCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTT‑GTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCATTCTCGTCTg                                                      <  1:389418/198‑1 (MQ=255)                                                                                                                                                     caaccaacCGCAGGTTAACATCTCGCTTGATAGCGCTGGTGGTAACATCATGACTAACTTCACTAAGGACAACATCGGCAAACCGATGGCACCCCTGTTTGTGGAGTACAAAGACAGCGGGAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCATCAGc  >  1:296901/1‑251 (MQ=255)                                                                                                                                                                                |                                                                                                                                                                                                                                ACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTTGTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGC  >  NC_000913/428506‑428904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑