| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 437,421 | G→A | L239F (CTC→TTC) | yajO ← | 1‑deoxyxylulose‑5‑phosphate synthase YajO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 437,421 | 0 | G | A | 96.0% | 55.4 / NA | 25 | L239F (CTC→TTC) | yajO | 1‑deoxyxylulose‑5‑phosphate synthase YajO |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/8); minor base C (1/0); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTG > NC_000913/437178‑437625 | cAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATATGTTCCGGCTTCAAAGTGATATCCCACGCGTTCAATAGCTCCTAAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCCACTTATGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTc > 1:444674/1‑251 (MQ=255) cAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCCAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCTCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCttatc > 1:506293/1‑237 (MQ=255) gTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACGACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGGTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTcc < 1:486513/250‑1 (MQ=255) cgAAGTTCCGATAATCGGTGAGGGAATGTACGGTTTACTCAACAACCAGGCCAGCGCAACTCGTGCTCGTGTCGCCCCCAGTTCTTCACTGAAGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGCAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTCGTTTCTCCCCACGGACGCGACAGACGGCCCCTTGCCAGCGGGCTCCATGGACTTACCGCCACGCcct > 1:504113/1‑251 (MQ=255) gAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTccc > 1:235141/1‑192 (MQ=255) gTTCCGATACTCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTCACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGCCGCGTCAGACGGcccc > 1:108623/1‑211 (MQ=255) nccntgcncnntnncccnaacaacCATGCCAGCGCAACTNNTGCTCGGGTNCCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTNTCATCGCTTNNTTNATAGANANNNTNNNCCNCCTTATCAGACACCAGGCGNGCNNTAGTTTNTCCCCACGGACGCGTCAGACGGcccc < 2:108623/175‑1 (MQ=255) ttACTCAACAACCAGGACAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:421808/1‑251 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGTGTCGACCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGCTCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTcccc > 1:424984/1‑117 (MQ=255) aacCAGGCNANNNCNNATTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACNGATCTGCGATCTGCGNGTNATTTNCATNGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTCGTTTCTCCCCACGGAc > 2:358003/1‑157 (MQ=255) aacCAGGCCAGCGCAACTGGGGCTCGTGGCGCCCCCAGTTCTTCACTGACGCCGGTGAACCGCTCTGCGATCGGCGCGTCAGTTGCATCGCTTTCTTAATAGAAATGTTTCCCCCCCATATCAGACACCAGTCGGGCGGTAGTTTCTCCCCACGGAc < 1:358003/157‑1 (MQ=255) ggCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATc > 1:497752/1‑250 (MQ=255) gCTCGTGTNGNNNCNNGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCANTTTCATCGCTTTCTTNATNGAAANTTTNCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCg > 2:345564/1‑160 (MQ=255) gCTCGTGTCGCCCCCATTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTGCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCg < 1:345564/160‑1 (MQ=255) ntCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACt > 1:126500/2‑251 (MQ=255) cTGACGCCTGTTAACCGCTCTGCGAACTGCGCGTCATTTTCATCGCTTTCTTTATAGAACTTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGc > 1:182850/1‑131 (MQ=255) gACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGGGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCGTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGcc < 1:456883/249‑1 (MQ=255) ngCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACTGCCCCTTGCCAGCGGGCTCCATGGCATTACCGCCACGCCCTCCTGATAACACATTGGTAGCATATCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGttc > 1:302609/2‑249 (MQ=255) cGCCTGTTAACCGCTCTGCGATCTGCGAGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt > 1:136962/1‑219 (MQ=255) gCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTAATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACAAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGc > 1:175755/1‑134 (MQ=255) ncTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTtc > 1:105708/2‑95 (MQ=255) ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAACCTGCATACTGACAAACGGCTCCCAGCCGTGCTg < 1:152537/251‑1 (MQ=255) cTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGc < 1:389512/132‑1 (MQ=255) aaCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAAATTTTTCCCCACCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTGGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGGTTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTg < 1:449800/251‑1 (MQ=255) gCGATCTGCGCGTCATTTTCCTCGCTTTCTTTATAGACATTTTTCCCCACCTTATCCGAAACCAGTCGTGCGGTAGTTTCTCCACACGGACGCGTc > 1:416237/1‑96 (MQ=255) tcgcgTCATTTTCATCGCTTTCTTTATAGAAATTTTGCCCCTCCTTATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGAGTCAGACGGCCCCTTGCCCGCGGGCTCCAGGTAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACTATAAATCAGATTGGAGTGATCCTGCATACTGACAAACTgcgc < 1:162221/216‑1 (MQ=255) | CAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTG > NC_000913/437178‑437625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |