Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 464,112 G→A G59D (GGC→GAC)  ybaV → helix‑hairpin‑helix 3 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,1120GA100.0% 65.9 / NA 23G59D (GGC→GAC) ybaVhelix‑hairpin‑helix 3 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

GATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTC  >  NC_000913/463904‑464350
                                                                                                                                                                                                                |                                                                                                                                                                                                                                              
gATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGGACCGGCGAAAGCCAGTGACGAAGAAGAGACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAg                                                                                                                                                                                                       <  1:175749/250‑1 (MQ=255)
                                                               aCCCTGTCCCTGGCCTGTGCCGGAATGGCTCATAGCGCGCGGGCGGCATCTTCTGTGGCGAAACCGACGGCGGTAGATACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCGGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGTGTCAGCATTAATAATGCCAGCGCGTAAGAGCTAGCCGGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGt                                                                                                                                        <  1:249331/250‑1 (MQ=255)
                                                                cccTGTCCCTTGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACTGACGGCGGTAGAAACCAAAGCGGAAGCTCCGGCAGCACAAAGGAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTggn                                                                                                                                                                                <  1:104710/209‑2 (MQ=255)
                                                                                  ccGGAATGTCTCATAGCGCGCTG‑CGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTgg                                                                                                                   >  1:22065/1‑251 (MQ=255)
                                                                                           ctcATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCt                                                                                                            >  1:187999/1‑250 (MQ=255)
                                                                                                         gcggcAGCTTCTGTGGCGAAACCGACGGCGGCAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCCGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTg                                                                                                                                                                             >  1:455398/1‑171 (MQ=255)
                                                                                                          cggcAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACACAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTgaag                                                                                                                                                                          >  1:448025/1‑173 (MQ=255)
                                                                                                                         cGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGANNCCCgggnn                                                                                                                                                                                                                                      >  2:8402/1‑96 (MQ=255)
                                                                                                                         cGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGc                                                                                                                                                                                                      <  1:8402/130‑1 (MQ=255)
                                                                                                                                       gTAGANNCCNNNGCNNAAGCTCCTGCAGCCCAAAGTAAANCAGCAGTACCGGCGAAAGCCAGTGACGAANANGNCACANNGGTNAGCGTTCCTANTGCCAGCGCGGAAGAGCTAGCCCGCGCGTTGAATGGCGTTGGCCTGAANANAGCGCAGGCGATTg                                                                                                                                                          >  2:376600/1‑160 (MQ=255)
                                                                                                                                       gTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCAAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAAGAATGCCAGCGCGGAAGATCTAGCCCGCTCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTg                                                                                                                                                          <  1:376600/160‑1 (MQ=255)
                                                                                                                                       gTAGAAACCAAAGCGGAAGCGCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATGAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAagan                                                                                                                                         <  1:59384/177‑2 (MQ=255)
                                                                                                                                                    cgaaaGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCGAGCCCGCGCGATGAAGTGCGTTGGCCGGAAGAAAGCGCATTCGATTGTCAg                                                                                                                                                      <  1:428123/148‑1 (MQ=255)
                                                                                                                                                        ngCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGACAGTGACGACGAAGACACCCGGGTCAGCCTTAATAAAGCCAGCGCGGAAGACCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGCAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCg                                                                                                                                >  1:101693/2‑169 (MQ=255)
                                                                                                                                                                 agcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACAACCGGGTCAGCATTAATAATGCCAGAGCGGAAGAGCTAGCCAGCGAGATGAATGGCGTTGGCCTGAAGAAAGAGCAGGCGATTGTCAGTTATCGCGAAGAGTCCGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATATGGCGGTAtt                                                      >  1:373190/1‑234 (MQ=255)
                                                                                                                                                                  gcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCAAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCTATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTg                                                                                                  <  1:67061/189‑1 (MQ=255)
                                                                                                                                                                     caAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATCATGCCAGCGCGGAAGAGCTAGCCCGAGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCATGTAATTAATTTGCAt                                 >  1:364875/1‑251 (MQ=255)
                                                                                                                                                                                   nnnaNCGNNGAAANCCAGTGACGAAGAAGACACCCGGGNCAGCATTAATAATGCCAGCGCGGAAGAGCTANCNCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTNATCGCGAAGANTACNNTCCGNTNANAACTGTGGAGGATCTAAAGCAGGTGCCGGNGATGGGCAATTCGCTGGTGGAACNNAANNNGGNNGTAtt                                                      <  2:373190/213‑1 (MQ=255)
                                                                                                                                                                                            gAAAGNNAGNNNCGNNGAAGACACCCGGGTCAGCATTAANAATGCCAGCGCGGCAGAGCTAGCCCGCGCNANGNCTGGNNTTGNCCTGAAGAAANCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTACAACTGNGNAGGATCTAAAGCAGGTGCCggg                                                                                             >  2:376135/1‑168 (MQ=255)
                                                                                                                                                                                            gAAAGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGTGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCggg                                                                                             <  1:376135/168‑1 (MQ=255)
                                                                                                                                                                                               aGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCgg                                                                                                                                                                                                                >  1:24488/1‑50 (MQ=255)
                                                                                                                                                                                               aGCCAGTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCgg                                                                                                                                                                                                                <  2:24488/50‑1 (MQ=255)
                                                                                                                                                                                                    gTGACGAAGAAGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGTAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGTATGGGCAATTCGCTGGTTGAACGTAATCGGGCGGTATTAACCCTGTAATGAAGTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTc  <  1:466157/251‑1 (MQ=255)
                                                                                                                                                                                                         gaagaaGACACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCCGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCgg                                                          >  1:416216/1‑190 (MQ=255)
                                                                                                                                                                                                                |                                                                                                                                                                                                                                              
GATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTC  >  NC_000913/463904‑464350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑