Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,816	G→A	G161D (GGT→GAT)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,816	0	G	A	100.0%	47.7 / NA	17	G161D (GGT→GAT)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

ATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTG  >  NC_000913/1109603‑1110030                                                                                                                                                                                                                      |                                                                                                                                                                                                                       aTACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATT‑TTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTgg                                                                                                                                                                                  <  1:6870/251‑1 (MQ=255)                     gAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGCTACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGcc                                                                                                                                                               >  1:451028/1‑251 (MQ=255)                                       gTCAAACGAATCACATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCgg                                                                                                                                                                                       >  1:140291/1‑209 (MQ=255)                                         cAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCcgc                                                                                                                                           >  1:13643/1‑251 (MQ=255)                                              gAATCAAATACAGCACGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCAGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGCTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGCATTTcc                                                                                                                                             >  1:383328/1‑244 (MQ=255)                                                                              ngCNNTGTTNAGCAGGACAAAGATCCGGTAAAAGNCCTTGGCTGTGCCGTTTTTAAAGTGCTTTACNCNATAAATAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCNCCTATTTCCGNGTTNNTGATNCNGNTCAGGTGTATGGCCTTTCGGCCCGCGGCCNGGCAATTGATACCGCCTTGCCATNNGGNNNAGNNTTTcc                                                                                                                                             <  2:383328/211‑1 (MQ=255)                                                                                cGATGTTCAGCAGGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACTATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGcaggtcacg                                                                                                                                                                                                             <  1:473647/146‑3 (MQ=255)                                                                                                                      gCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGATCAGGGCCAGCTATATCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACAGCCTTGCCATAGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCg                                                                                                                  >  1:337337/1‑199 (MQ=255)                                                                                                                                          cTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTgn                                                                                                                                                                                             <  1:301601/104‑2 (MQ=255)                                                                                                                                          cTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGc                                                                                                                                                                                             >  2:301601/1‑104 (MQ=255)                                                                                                                                                       aCAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCAGCGTGATTGATGAAGGTCAGGTTTATGGc                                                                                                                                                                                                     >  1:466631/1‑83 (MQ=255)                                                                                                                                                          ncAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGcc                           >  1:48141/2‑250 (MQ=255)                                                                                                                                                                  aaaaaNGATGANANNGTCAGCATGCTCGGGGCCANCTATTTCCGCGTGATNGATGNAGGTCNGGntn                                                                                                                                                                                                          >  2:207867/1‑66 (MQ=255)                                                                                                                                                                  aaaaaCGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAGCCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg                                                        <  1:207867/213‑1 (MQ=255)                                                                                                                                                                           gAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGc                                                                                                                                                                                                                     >  1:293266/1‑47 (MQ=255)                                                                                                                                                                           gAAATCGTCAGCATGCTCGGGGCCAGCTANTTCNNCGNNATNGATGc                                                                                                                                                                                                                     <  2:293266/47‑1 (MQ=255)                                                                                                                                                                                   cAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGATGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATGCGTGGTTATGCCAGGGCGTGACACGGTTGTGGAtgtg  <  1:193139/250‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                       ATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTG  >  NC_000913/1109603‑1110030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑