Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,369,455	G→A	K21K (AAG→AAA)	pspD →	phage shock protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,369,455	0	G	A	100.0%	43.7 / NA	15	K21K (AAG→AAA)	pspD	phage shock protein D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/13);  total (2/13)

GCTTGATCCAATGCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGG  >  NC_000913/1369213‑1369656                                                                                                                                                                                                                                                   |                                                                                                                                                                                                          gcTTGATCCAATGCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTAct                                                                                                                                                                                                   <  1:438082/251‑1 (MQ=255)  cTTGATCCAATGCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGCCCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGAAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTActt                                                                                                                                                                                                  >  1:507138/1‑251 (MQ=255)     gATCCAATGCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCTAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACTTACCCTTTTCCGTCAACTGTGAGGAAAGTTATTAATACTCGCTGGCAACATGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTActtctt                                                                                                                                                                                               <  1:191567/251‑1 (MQ=255)             gCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCan                                                                                                                                                                                        <  1:47997/250‑2 (MQ=255)                       aTGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGc                                                                                                                                                                             <  1:336242/251‑1 (MQ=255)                                   gAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGCGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTan                                                                                                                                                                                             <  1:97618/223‑2 (MQ=255)                                                   gcagcGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGTGCAAAAGGTAAATCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCggg                                                                                                                                                                     <  1:431813/231‑1 (MQ=255)                                                                      aGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGAGAAAATCAGTTGCTCGc                                                                                                                                   <  1:415013/246‑1 (MQ=255)                                                                                                  aCGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAAGTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTTCTGTCTGTGGCGCTg                                                                                                  <  1:388087/251‑1 (MQ=255)                                                                                                                                                 gTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTTGCAACATGCCGGTCAAAAGGTAAAGCCTGGTGTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGTCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTc                                                                                                                                     <  1:167942/169‑1 (MQ=255)                                                                                                                                                                          tgAGGAAAGTTATGAATACTCGCTGGTAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTg                                                                                                                                        <  1:357570/141‑1 (MQ=255)                                                                                                                                                                                           aCTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCAGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCg          <  1:493956/250‑1 (MQ=255)                                                                                                                                                                                                  ggCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAAATGGTACTTCTTACAGCACTGCGCTATGGCCAGGCGGGTGTGGCGGGCTGGGGGGAAAACTCAGGTGCTCGCCGACCGCCTAAAATGTTGCTGGGTGTGGGGCTGGGAACGCCGGTTAGGAGGGATGCTCATTAACTGGACCAGAGTTTTAAAAGGTGAGGGGGGTTTCGGAAAAATTGTTATATCTCAGGCGGATAatgg  >  1:481271/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAAATTAGCAGGCAAACTGGTCCTTCTTACCGCACTGCGCTATGGCCCGGCTGGTTTGGCGGGCTGGTCGATAAAAGCAGTTGCGCTCTGACCGCTTAAAATTTTGCTTGCTTTGGCGCTGGaa                                                                                               <  1:409618/124‑1 (MQ=255)                                                                                                                                                                                                                                            cAGGCAAACTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGc                                                                                                                                                           <  1:360105/56‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                          GCTTGATCCAATGCCGGACAACATGGCCTTTGGTGAGCAGCTACCTTCCAGCAGCGAATTGCTGGATGAAGTCGACCGTGAACTGGCGGCAAGTGAAACGCGTTTACGCGAGATGGAACGTTATGTCACTTCCGATACTTTCACGTTACGTAGCCGTTTCCGTCAACTGTGAGGAAAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGG  >  NC_000913/1369213‑1369656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑