Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,259	G→A	A196T (GCG→ACG)	glaR →	DNA‑binding transcriptional repressor GlaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,259	0	G	A	100.0%	24.1 / NA	12	A196T (GCG→ACG)	glaR	DNA‑binding transcriptional repressor GlaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)

GCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGT  >  NC_000913/2796095‑2796489                                                                                                                                                                     |                                                                                                                                                                                                                                       gCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGAGGGGCGTTATAGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCACCACCAGACCATGACTGCGGCGGTACTGGCGCGAGATACCACGCGCGCCCGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCCATTAAATACTCTTc                                                                                                                                                  >  1:86865/1‑251 (MQ=255)                                                                gATTTATCTGGCTGCGGCGAACGGTGCTTTCGGGGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGGGGCGGGACTGGGGCGAGATACCACGCGCGGCAGGGAGTTAATGCGCCAGCATTTACTGACg                                                                                                                                                                                                  <  1:442726/140‑1 (MQ=255)                                                                             gcggcgAACGGTGCTTTCGGTGGAAATGCTGGAGGATACACACGATCAGCACCAGACCCTGACTCCGGCGGTACTTGCGCGAGATACCACGCGCGCCCGTGAGTTAATGCGCCAGCCTTTACTGACGCCCATTTCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGCATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTCGCATCGGTTTATTCg                                                                                    >  1:478322/1‑237 (MQ=255)                                                                              cggcgAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTAt                                                                     >  1:107597/1‑251 (MQ=255)                                                                                 cgAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATc                                                                                                              >  1:38112/1‑207 (MQ=255)                                                                                                         cTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCt                                          >  1:401183/1‑251 (MQ=255)                                                                                                           ggaggaTAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCg                                                 <  1:389789/242‑1 (MQ=255)                                                                                                                                ccAGGCCCTGACTGCGGCNTTACTGGCGCGAGATACCACGCGCGCCAGTGNGNTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGANGGCTGGCAATNAACNNCTCTNCNGNAATACGCAACACTTGCCCCGGATAAATTTNATCCGGGCTTTTTAGCATCGGTTNNTTNNNTTNNAAGAt                                                                          <  2:363895/196‑1 (MQ=255)                                                                                                                                ccAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCAAGCGCGCCAATGAGTTAATGCGCCCCCATTTTCTGACGCCCATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCTCTTCGCAGAt                                                                          >  1:363895/1‑196 (MQ=255)                                                                                                                                                 cggTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATACAGCCGGCGCTGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGACAGACTTAACGGt  >  1:442729/1‑251 (MQ=255)                                                                                                                                                  ggTACTGGCGCGAGATACCACGCGCGCCAGTGAGTTAATGAGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATTGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGCCCTGTTTGGGAATGGCACTCACAGTGTCGCCAAACTTAACGGt  >  1:349463/1‑250 (MQ=255)                                                                                                                                                          cgcgAGATACCACGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATCCGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATg                               >  1:408260/1‑213 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                                                       GCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGT  >  NC_000913/2796095‑2796489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑