| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,416,797 | G→A | P197L (CCT→CTT) | recE ← | exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,416,797 | 0 | G | A | 96.3% | 72.8 / NA | 27 | P197L (CCT→CTT) | recE | exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/14); minor base C (1/0); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GACGTCAGATCGTGTACGAAACCTTCGCCGCGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGT > NC_000913/1416599‑1417009 | gACGTCAGATCGTGTACGAAACCTGCGCCGCGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTAGCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTtgtgn < 1:49050/240‑2 (MQ=255) cGAAACCTTCGCCGCGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAg > 1:412980/1‑226 (MQ=255) tCGCCGCGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCTCGAATTTCCAGCGGGTCGAGCAGGCTg < 1:138141/249‑1 (MQ=255) gccgcGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCATTCCTCCACTCTTTTGTACA‑CAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGttt > 1:212386/1‑251 (MQ=255) cgATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTtgtg < 1:39266/251‑1 (MQ=255) gccAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAg > 1:17933/1‑239 (MQ=255) gCCGTNNGANNNATNNGTGAAACACGATTACCCTTCATCNACTCTTTTGT‑CAGCAGTCCTCGATCGGTGNANTNAGCGNNCAGNTATGCTTCGANAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACNTNGTCAGTGTCACGAACCAGTTtg > 2:364550/1‑168 (MQ=255) gCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTGATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTtg < 1:364550/168‑1 (MQ=255) gATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTAGATCGGTGTCGTCAGCGTTCCGGTCTGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGACTTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAAtg > 1:219448/1‑250 (MQ=255) gtgtgCAACACGATTACCCTTCATCCACTCTTTTGT‑CAACCGTCCTCGATCGGTGTAGTCAGCGTTCCGGTATGCATCGAAAAAAGCAGTTATAAGTCCAAGGTTTGAATTACCACGATTAGGGAAAACTTTGTCAGTGTCACGACCCAGTTTGTgg > 1:364614/1‑157 (MQ=255) gtgtgAAACACNANTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGNGTTCAGGTATNCTTNNAAAANANCNGTTATCAGTCCCAGGTTTGAATTACCAAGNTTAGGGAAAACTTTGTCAGTGTCNNGANNNAGNNTGTgg < 2:364614/157‑1 (MQ=255) tgtgAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGaaa > 1:24489/1‑126 (MQ=255) cacGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGATTTCCCAAGTTTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGAAAAAGCCGGTAGTTCTTCAGCCCGAGCAATTGGTAATGCCTgg > 1:356426/1‑251 (MQ=255) aCCCTTCATCCGCTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCNg < 1:394914/250‑1 (MQ=255) cACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTAACAAGATTAggg > 1:216123/1‑99 (MQ=255) nntttGT‑CNNCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGaaa < 2:24489/96‑1 (MQ=255) gcagTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCa > 1:173814/1‑171 (MQ=255) ggTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAAc < 1:254596/78‑1 (MQ=255) gTCAGCGTTCAGGGATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGATCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTg > 1:41660/1‑249 (MQ=255) ttCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGc < 1:137690/173‑1 (MQ=255) gTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGa > 1:420881/1‑250 (MQ=255) gCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGGAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATgg < 1:379520/250‑1 (MQ=255) cGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTTCCAAGTTTAGGGAAAACTTTTTCAGTGTCACGAACCAGTTTGTGTAGTTCGGGAATTTCCAGCGGGTCGTGCCGGCTGGTTTTGTGGGCAACCGCCAGGGTAGTAACAGTCGGTCGTTCTTCAGCCCGGGCGATGTGTAATGCCTCGAGTACGTCGCGTTTAACGTGCGTTACCGGTTCTTCGCTGCCGTGTTGATCATGCCGTCGAATGGGccgt < 1:167522/251‑4 (MQ=255) gTCCCAGGTTTGAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTAGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAAc > 1:359428/1‑181 (MQ=255) gTCCCAGGTTTGAATTACCAAGAGTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGNTGGNTTTGNGGNAAACAGCCAGGGCAGNAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGANNCNNNCNCGTGAAAc < 2:359428/181‑1 (MQ=255) gAATTACCAAGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGTGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCTTTAAACGTGCGTTACCGGTTTTTCGc < 1:384722/190‑1 (MQ=255) aCCAANNTTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAg < 2:17933/95‑1 (MQ=255) | GACGTCAGATCGTGTACGAAACCTTCGCCGCGATCGGTGAGGTTTCCGCCGCCAGCATTAGCACCGGAAGCCGTGCGAGTGATGTGTGAAACACGATTACCCTTCATCCACTCTTTTGT‑CAGCAGTCCTCGATCGGTGTAGTCAGCGTTCAGGTATGCTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGT > NC_000913/1416599‑1417009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |