Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,643,308	G→A	F625F (TTC→TTT)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,643,308	0	G	A	100.0%	49.6 / NA	18	F625F (TTC→TTT)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

AGCGGGCCGCCAGAACAGATAAGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGGCACCACGGCAACCAGTTTCTTGATTTCTGCCAGAGTTTCGAGGATTTTTGCCCCCTGATCCG  >  NC_000913/3643066‑3643483                                                                                                                                                                                                                                                   |                                                                                                                                                                                ccgatcgcgccAGAACAGATAAGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCAtn                                                                                                                                                                         <  1:121553/244‑2 (MQ=255)                       ggCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAAGGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCCCGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCGCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCca                                                                                                                                                    <  1:20236/250‑1 (MQ=255)                           ccGTCTCCGGTGCCTGTTTCATCAATTAAACAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCAGCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCAATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCGGCCGCCAGTACGTCAGCCCACAGGt                                                                                                                                               <  1:390176/251‑1 (MQ=255)                                                                             aGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACAACGGAAGCGTTTGAACAGATCCATCGGCCCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAACGCGCGAAAAAGCATCTGCCGCCAGTACGt                                                                                                                                                           >  1:288858/1‑189 (MQ=255)                                                                                    aaTGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCggg                                                                                       <  1:201655/250‑1 (MQ=255)                                                                                          gaaaTGCTCCAGCTTCGCATCCAGCTGCGGTTCATGCCCACGGAAGCTTTGGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGc                                                                                <  1:333695/249‑1 (MQ=255)                                                                                                                      ggctCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGc                                                                                                                            >  1:82453/4‑179 (MQ=255)                                                                                                                       gTTCACGACCACGGAAGCGTGTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATTTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAAGTCCCTCTGCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTgtagt                                                                                                                                      <  1:338465/168‑1 (MQ=255)                                                                                                                           ccGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCTCCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGGCACCACGGCAACCAGtt                                               <  1:248091/250‑1 (MQ=255)                                                                                                                                                cAGATCCATCGGCTTTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCACAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGGCACCACGGCAACCAGTTTCTTGATTTCTGCCAGAGttt                          <  1:242345/251‑1 (MQ=255)                                                                                                                                                                tctGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACGGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATAGGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGTCACCACGGCAACCAGTTTCTTGATTTCTGCCAGAGTTTCGAGGATTTTTGccc           <  1:375044/250‑1 (MQ=255)                                                                                                                                                                   gAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAACTACCCTCTTCCTCAAAACGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGACGAACATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGGCACCACGGCAACCAGTTTCTTGATTTCTGCCAGAGTTTCGAGGATTTTTGCCCCCTg       >  1:295547/1‑251 (MQ=255)                                                                                                                                                                        gCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAACGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGAACGGAGATGGCACCACGGCAACCAGTTTCTTGCTTTCTGCCAGAGTTTCGAGGATTTTTGCCCCCTGATCCg  >  1:332158/1‑251 (MQ=255)                                                                                                                                                                           aCGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGCCGGAGATGGCACCACGGCAACCAGTTTCTTGATTTCTg                                   >  1:160480/1‑215 (MQ=255)                                                                                                                                                                                          gtCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAAAAAGCATCTGCCGCCAGTACGTCCGCCCACAGGTAGCTGAAGTAACCTGCGGCATAACCACCGGCGAAAATATGGNTGANNNNNTgnn                                                                                         >  2:317077/1‑144 (MQ=255)                                                                                                                                                                                                             gTTTCACGGTTGAAAATGCCCTCTTAATCAAAGCGCGAAAAAGCATCTGCCGCCCGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGaa                                                                                                 >  1:137814/1‑119 (MQ=255)                                                                                                                                                                                                                 cACGGTTGAAAATGCCCTCTTCCGCAAAGCGCGAAAAACCATCGGCCGCCAGTACTTCAGCCGTGAGGGAGTTGGAGTAACCTGCGGCATAACCACCGGCGAAAATAGGGCTGAAAGCGTGCGGGAAACGGCCCCAGGAc                                                                        <  1:330869/140‑1 (MQ=255)                                                                                                                                                                                                                                         naaaGCGCGAAAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACATGCGGCATAACCACCGGc                                                                                                                >  1:62643/2‑76 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                AGCGGGCCGCCAGAACAGATAAGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGCTGAAAGCGTGCGGGAAACGGCCCCAGGACGGAGATGGCACCACGGCAACCAGTTTCTTGATTTCTGCCAGAGTTTCGAGGATTTTTGCCCCCTGATCCG  >  NC_000913/3643066‑3643483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑