| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 402,739 | G→A | L331L (TTG→TTA) | phoA → | alkaline phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 402,739 | 0 | G | A | 94.4% | 42.2 / NA | 18 | L331L (TTG→TTA) | phoA | alkaline phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/8); minor base C (1/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTGTTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCGG > NC_000913/402572‑402965 | ccccTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCTAATCCTTGTgg < 1:399259/251‑1 (MQ=255) tGCCAGTGCGCTGGCTAGGACCGCAAGCAACGAACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGAtctc > 1:169688/1‑245 (MQ=255) cTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAAAGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCCTTTTCCTGCaa > 1:505043/1‑160 (MQ=255) aGGACCGAATGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATTAGAAATGTTTTTTCTTGCAAGTTGAAGGTGCGCCAATCGATGATCCGGATCATGCGGCGAATCCGTGTGGGCAAATTCGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGgc < 1:380887/249‑1 (MQ=255) aCCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCACATCCGCATCGTAATGACCGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGCGAAAGGCTTTTTCCTGTAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTgg > 1:497777/1‑251 (MQ=255) gTACCATGGCAATATCGATGAGCACGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTaaa > 1:173889/1‑250 (MQ=255) tACCATGGCAATATCGATAAGCCCGCAGGCACCTGTAAGCAAAATCCGCAACGTCATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGACATTGAAttattg > 1:460300/1‑108 (MQ=255) aCCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGGCCGACAAAGCCATTGAATTATTGAGTAAAATTGAGAAAGGCTTTTTCCTGCAAGGTGAAGGt < 1:181362/146‑1 (MQ=255) ccATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAagn < 1:149387/251‑2 (MQ=255) cGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTCTACCAACCCTGGCGCAGATGACCGCAAAACCCCTTGAATTCTTGAGTAAAATTGAGAAAGGCTTTTTCCTGCAAGCTGACGGTGCGTCAATCGCTAAACAGGATCATGCTGCGAATCATTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGACTTCGCTAAAAaggag > 1:472475/1‑232 (MQ=255) cAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTCCCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGAAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTc > 1:361510/1‑251 (MQ=255) gTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCgn < 1:305293/190‑2 (MQ=255) gTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGANCATNNNNNGann > 2:305293/1‑144 (MQ=255) ccAACTCCGCAACGTAATGACAGTGTACCAACCCTGGCGCTGATGACCGACAAATACATTGAATTATTTAGCAAAAATGAGAAAGGCTTTTTCCTGCAAGTGGAAGGTGCGTCAATCGATAAACAGTATCATGCATCGAATCCTTGTGGGCAAATTGGCGAGACGTTCGGTCTCGATGAACCCGTACAACGGGCGCGGTAAGTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCa < 1:322274/238‑1 (MQ=255) cAGTGTACCAACCCTGGCGCAGATGACCGACACAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCACGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTAGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGCttgttg > 1:168753/1‑251 (MQ=255) cTGGCGCAGATGACCGACAAAGCCATTGAATTATTTAGTAAAAATGAGAAAGTCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGGGGGCAAATTGGCGAGACTGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAATGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATAc < 1:343925/248‑1 (MQ=255) naTGACCGACAAAGCCATTGAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCACGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTGCAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCgg > 1:404359/2‑251 (MQ=255) naaTTATTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGa > 1:297998/2‑68 (MQ=255) gAATTATTGAGTAAAAATGAGAAAGGCTTTTTCCGGCAAGTTGAAGGTGCGTCAATCGATAAACAGGa < 2:297998/68‑1 (MQ=255) | CCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTGTTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCGG > NC_000913/402572‑402965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |