Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,038	G→A	H97Y (CAT→TAT)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,038	0	G	A	100.0%	32.5 / NA	15	H97Y (CAT→TAT)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

TACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGT  >  NC_000913/699801‑700264                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                   tACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGcc                                                                                                                                                                                                                        <  1:191542/250‑1 (MQ=255)           gAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGAACACAACGCGCCACCCGCGGGATAACACCCGCGCCCGTGGGTGTCCGGATTGGTGGCGGTAAAACGTGCCCAGTTCGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATAAGTGCGCCTTCGCccacc                                                                                                                                                                                                              >  1:453014/1‑250 (MQ=255)                         cGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACAGACTCTTTCTNg                                                                                                                                                                                               <  1:396382/251‑1 (MQ=255)                                                                                cccGTGGGTGTCCGGATTGGTGGCGAGAAAACGTGCAGCGTTAGCGACGAAATAGGCTGCTTGATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTGCACATCGGTAATATGGAAACCGTCTTTGTACAGGTGATAGATCAGTTCGCCGTCGCCGAGCACATACTCGTTCCTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGcc                                                                                                                                                              <  1:204314/229‑1 (MQ=255)                                                                                                                gTGCACCGTTGGCGACGAANTNGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACNATCACGAAATNATGNNTCTCNTNGNTAATAGTGAAACCGGCTTTGTTCAGTTCANAAATCAGTGCGCCTTCGCCCACCNNATNNNCTNNCTTGc                                                                                                                                                                                              <  2:365759/165‑1 (MQ=255)                                                                                                                gTGCACCGTTAGCGACGAAATAGGATGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTCATAGTGACACCGGCTTTGTACAGATCATAAATAAGTGCGCCTTCGCCCACCACATACGCTTTCTTGc                                                                                                                                                                                              >  1:365759/1‑165 (MQ=255)                                                                                                                  gCACCGTTAGCGACGAANTNGGCTGCTGTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACNATCACGAAATNAGGNNTCACNTNGNTAATAGTGAAACCGGCTTTGTACAGTTCANAAATCAGTGCGCCTTCGCCCACCNNATNNNCTNNCTTGc                                                                                                                                                                                              <  2:380006/163‑1 (MQ=255)                                                                                                                  gCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTCATAGTGACACCGGCTTTGTACAGTTCATACATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGc                                                                                                                                                                                              >  1:380006/1‑163 (MQ=255)                                                                                                                      cGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAACTCAGGGTTCACCTCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCAt                                                                                                                                                            >  1:403719/1‑193 (MQ=255)                                                                                                                           gCGACGAAATAGGCTTCTTTATGCAGCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTATGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAg                                                                                             <  1:507921/251‑1 (MQ=255)                                                                                                                            ngacgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGCACTCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGcc                                                                                               >  1:62016/2‑248 (MQ=255)                                                                                                                                                                        cgcgTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCGTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGttn                                                                 <  1:44750/234‑2 (MQ=255)                                                                                                                                                                          cgTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCCCATACGCTTTCTTGCCTTCCTGGCGACg                                                                                                                                                                                 >  1:69362/1‑120 (MQ=255)                                                                                                                                                                                                                tAATAGTGAAACCGGCTTTGTACCGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGAGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCgg                                                >  1:155945/1‑211 (MQ=255)                                                                                                                                                                                                                                        nttCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGt  >  1:147991/2‑233 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                   TACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGT  >  NC_000913/699801‑700264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑